Abstract：
Common vetch (Vicia sativa L.) is widely planted as forage, green manure and food. Phosphate (Pi) deficiency is an important constraint for legume crop production. In this study, P-deficiency tolerance in 40 common vetch collections was evaluated under hydroponic condition. The collections were clustered into three groups based on the tolerance level. Physiological responses to P-deficiency in two tolerant collections (418 and 426) in comparison with one sensitive collection (415) were investigated. Greater growth inhibition was observed in sensitive collection compared with two tolerant collections, although the inorganic phosphorus (P) content in sensitive collection was higher than those in tolerant collections. The internal and external purple acid phosphatase activity in plants showed no significant difference between 418 and 415 under low phosphate condition. Transcriptomic analysis in the tolerant collection 426 in response to Pi starvation showed that many common adaptive strategies were applied and PHOSPHATE STARVATION RESPONSE (PHR)-related Pi signaling and transporter genes were altered. VsPHT1.2 had the highest expression level in root among all VsPHT1s, and it was remarkably upregulated after short time of P-deficiency treatment in tolerant collections compared with sensitive collection. In conclusion, common vetch response to P starvation by altering the expressions of core genes involved in Pi transport and signaling, and the elevated expression of VsPHT1.2 gene might contribute to higher Pi acquisition efficiency in P-deficiency tolerant collections.
摘要：
普通豌豆（ViciasativaL.）被广泛种植为牧草，绿肥和食物。磷酸盐（Pi）缺乏是豆类作物生产的重要制约因素。在这项研究中,在水培条件下评估了40种常见vetch采集的P缺乏耐受性。根据耐受性水平将集合分为三组。与一个敏感集合（415）相比，研究了两个耐受性集合（418和426）中对P缺乏的生理反应。与两个耐受集合相比，在敏感集合中观察到更大的生长抑制，尽管敏感集合中的无机磷（P）含量高于耐受集合中的无机磷（P）含量。在低磷酸盐条件下，植物体内和外部的紫色酸性磷酸酶活性在418和415之间没有显着差异。响应于Pi饥饿的耐受性集合426中的转录组分析显示应用了许多常见的适应性策略,并且磷酸盐饥饿反应(PHR)相关的Pi信号传导和转运蛋白基因被改变。在所有VsPHT1s中，VsPHT1.2在根中的表达水平最高，与敏感集合相比，在耐受集合中经过短时间的P缺乏治疗后，它明显上调。总之,通过改变参与Pi转运和信号传导的核心基因的表达，对P饥饿的共同反应，VsPHT1.2基因的表达升高可能有助于P缺乏耐受集合中更高的Pi获得效率。
