Abstract：
Regenerative endodontics aims to restore pulp tissues, thus preserving the vitality of the tooth. One promising approach involves the utilization of decellularized human dental pulp (DHDP) as a scaffold repopulated with Wharton\'s Jelly mesenchymal stem cells (WJMSCs). This study aimed to regenerate pulp tissues using DHDP and WJMSCs following pulpectomy in mature canine teeth of a feline animal model and to investigate the histological features of the regenerated pulp. A 12-month-old male domestic shorthaired felines were used as subjects. Teeth were categorized into untreated (Group 1), pulpectomy with mineral trioxide aggregate (MTA) (Group 2), and pulpectomy with DHDP-repopulated scaffold and MTA (Group 3). The animals were sacrificed six weeks post-intervention. H&E and immunohistochemistry using anti-collagen type 1 and laminin antibodies were used to stain the tissue sections. Histological examinations presented pulp-like tissues in Group 3, with tissue components similar to the structures found in Group 1. Immunohistochemical analysis demonstrated the presence of collagen type I and laminin within the regenerated tissues. The root canals of teeth in Group 2 were devoid of pulpal tissue. DHDP with WJMSCs can potentially be used for pulp regeneration, supporting the modality for developing new clinical protocols in stem cell therapy.
摘要：
再生牙髓旨在恢复牙髓组织，从而保持牙齿的活力。一种有希望的方法涉及利用脱细胞的人牙髓（DHDP）作为用沃顿的果冻间充质干细胞（WJMSC）重新填充的支架。这项研究旨在在猫科动物模型的成熟犬齿中进行牙髓切除术后，使用DHDP和WJMSCs再生牙髓组织，并研究再生牙髓的组织学特征。使用12个月大的雄性家养短毛猫作为受试者。牙齿被归类为未经治疗（第1组），使用三氧化矿物质聚集体（MTA）进行牙髓切除术（第2组），和使用DHDP再填充支架和MTA的牙髓切除术（第3组）。在干预后6周处死动物。使用H&E和使用抗1型胶原和层粘连蛋白抗体的免疫组织化学染色组织切片。组织学检查显示第3组的牙髓样组织，其组织成分与第1组的结构相似。免疫组织化学分析证明再生组织中存在I型胶原和层粘连蛋白。第2组的牙齿根管没有牙髓组织。DHDP与WJMSC可用于纸浆再生，支持开发新的干细胞治疗临床方案的方式。
