Abstract：
Saccharomyces cerevisiae has become a key microbial cell factory for producing biofuels, recombinant proteins, and natural products. The development of efficient cell factories relies on the precise control and fine-tuning of gene expression, underscoring the pivotal role of promoters in pathway engineering. However, natural promoters often have limited transcriptional capacity and thus fall short of the metabolic engineering requirements. This chapter provides protocols and guidelines for constructing and evaluating synthetic promoters in S. cerevisiae. Moreover, these protocols are applicable for creating and testing various synthetic promoters in other host systems.
摘要：
酿酒酵母已成为生产生物燃料的关键微生物细胞工厂,重组蛋白，和天然产品。高效细胞工厂的发展依赖于基因表达的精确控制和微调,强调启动子在途径工程中的关键作用。然而,天然启动子通常具有有限的转录能力，因此达不到代谢工程的要求。本章提供了在酿酒酵母中构建和评估合成启动子的方案和指南。此外，这些方案适用于在其他宿主系统中创建和测试各种合成启动子。
