Abstract：
Macrophomina phaseolina is a wide host ranged soil-borne fungal plant pathogen. It infects more than 500 host plant species belonging to 100 families. Many important oil-seed and leguminous crops are known to be attacked by this devastating plant pathogen. In the present study, antifungal potential of flowers of a leguminous tree Acacia nilotica subsp. indica, was assessed against this pathogen through bioassays guided fractionation. Initially, methanolic extracts of 1 %-5 % of leaf, flower, root-bark and stem-bark of the plant species under consideration were evaluated for their antifungal potential against the target pathogen. Among these, the best antifungal activity was shown by flower extract. The reduction in growth of the test fungal strain was 27-49 %, 4-40 % and 2-27 % due to flower, root-bark and leaf extracts, respectivey, over control. Flower extract was partitioned using n-hexane, chloroform, ethyl acetate and n-butanol as the solvents. Bioassays guided study of these fractions of methanolic extract of flower revealed that high antifungal potential was shown by n-hexane and chloroform fractions against M. phaseolina causing 26-53 % and 28-50 % decline in fungal biomass, respectively, as compared to that of control. GC-MS analysis of chloroform fraction revealed the presence of 27 compounds in this fraction. Among these cyclopentanol,-1-methyl (10.93 %) was the predominant compound followed by methyl, 4,4-dimethyl butanoate (7.04 %), 1-pentanol (6.80 %), 2-propanol, 1-cyclopropyl (6.11 %), 1H,imidazole-4-5-dihydro-2-methyl (5.93 %), trichloroethane (5.91 %), carbonic acid-ethyl hexyl ester (4.59 %), 1,4-butandiol,2,3-bis(methylene)- (4.54 %) and [S]-3,4-dimethyl pentanol (4.48 %).
摘要：
Macrophominaphaseolina是一种寄主范围广泛的土壤传播真菌植物病原体。它感染100科的500多种寄主植物物种。已知许多重要的油料作物和豆科作物受到这种破坏性植物病原体的攻击。在本研究中,豆科树金合欢亚种花的抗真菌潜力。印度,通过生物测定指导的分级分离对这种病原体进行了评估。最初，1%-5%叶的甲醇提取物，花,评估了所考虑的植物物种的根皮和茎皮对目标病原体的抗真菌潜力。其中,花提取物表现出最佳的抗真菌活性。测试真菌菌株的生长减少了27-49％，4-40％和2-27％由于花，根皮和叶提取物，尊敬的,过度控制。花提取物使用正己烷进行分配，氯仿,乙酸乙酯和正丁醇作为溶剂。对花朵甲醇提取物的这些部分的生物测定指导研究表明，正己烷和氯仿部分对M.phaseolina显示出高抗真菌潜力，导致真菌生物量下降26-53％和28-50％，分别,与控制相比。氯仿级分的GC-MS分析显示在该级分中存在27种化合物。在这些环戊醇中，-1-甲基（10.93％）是主要化合物，其次是甲基，4,4-二甲基丁酸酯（7.04%），1-戊醇(6.80%),2-丙醇,1-环丙基(6.11%),1H,咪唑-4-5-二氢-2-甲基（5.93%），三氯乙烷(5.91%),碳酸-乙基己酯(4.59%),1,4-丁二醇,2,3-双(亚甲基)-(4.54%)和[S]-3,4-二甲基戊醇(4.48%)。
