PDF(Pubmed)
Abstract：
A wide range of virus-like particles (VLPs) is extensively employed as carriers to display various antigens for vaccine development to fight against different infections. The plant-produced truncated variant of the hepatitis E virus (HEV) coat protein is capable of forming VLPs. In this study, we demonstrated that recombinant fusion proteins comprising truncated HEV coat protein with green fluorescent protein (GFP) or four tandem copies of the extracellular domain of matrix protein 2 (M2e) of influenza A virus inserted at the Tyr485 position could be efficiently expressed in Nicotiana benthamiana plants using self-replicating vector based on the potato virus X genome. The plant-produced fusion proteins in vivo formed VLPs displaying GFP and 4M2e. Therefore, HEV coat protein can be used as a VLP carrier platform for the presentation of relatively large antigens comprising dozens to hundreds of amino acids. Furthermore, plant-produced HEV particles could be useful research tools for the development of recombinant vaccines against influenza.
摘要：
广泛的病毒样颗粒（VLP）被广泛用作载体，以展示用于疫苗开发的各种抗原，以对抗不同的感染。植物产生的戊型肝炎病毒(HEV)外壳蛋白的截短变体能够形成VLP。在这项研究中,我们证明，包含截短的HEV外壳蛋白与绿色荧光蛋白（GFP）或插入Tyr485位置的甲型流感病毒基质蛋白2（M2e）的细胞外结构域的四个串联拷贝的重组融合蛋白可以在烟草中有效表达。基于马铃薯病毒X基因组的自我复制载体。植物产生的融合蛋白在体内形成展示GFP和4M2e的VLP。因此,HEV外壳蛋白可用作VLP载体平台,用于呈递包含数十至数百个氨基酸的相对大的抗原。此外，植物生产的HEV颗粒可能是开发抗流感重组疫苗的有用研究工具.
