PDF(Pubmed)
Abstract：
In China, the Liaodong Peninsula is an important growing area for blueberries because of the high organic matter content in the soil, the abundance of light, and the large temperature difference between day and night. However, the low temperature and relative humidity of the air during the winter and early spring in the Liaodong Peninsula are the main reasons for the damage to blueberry plants. Here, we documented the transcriptome and proteome dynamics in response to cold stress in three blueberry cultivars (\'Northland\', \'Bluecrop\', and \'Berkeley\'). Functional enrichment analysis indicated that many differentially expressed genes (DEGs) and differentially abundant proteins (DAPs) were mainly involved in the pathways of protein processing in the endoplasmic reticulum, the glutathione metabolism pathway, and ribosomes. We identified 12,747 transcription factors (TFs) distributed in 20 families. Based on our findings, we speculated that cold tolerance development was caused by the expression of calcium-related genes (CDPKs and CMLs), glutathione proteins, and TFs (NAC, WRKY, and ERF). Our investigation found that three cultivars experienced cold damage when exposed to temperatures between -9 °C and -15 °C in the field. Therefore, the cold resistance of blueberries during overwintering should not only resist the influence of low temperatures but also complex environmental factors such as strong winds and low relative humidity in the air. The order of cold resistance strength in the three blueberry cultivars was \'Berkeley\', \'Bluecrop\', and \'Northland\'. These results provide a comprehensive profile of the response to cold stress, which has the potential to be used as a selection marker for programs to improve cold tolerance in blueberries.
摘要：
在中国,辽东半岛是蓝莓的重要产区,充足的光，昼夜温差大。然而,辽东半岛冬季和早春期间空气的低温和相对湿度是蓝莓植物受损的主要原因。这里,我们记录了三个蓝莓品种对冷胁迫的转录组和蛋白质组动态响应(“北国”，\'Bluecrop\',和\'Berkeley\'）。功能富集分析表明，许多差异表达基因（DEGs）和差异丰度蛋白（DAPs）主要参与内质网蛋白质加工途径。谷胱甘肽代谢途径，和核糖体。我们确定了12,747个转录因子（TFs）分布在20个家族中。根据我们的发现,我们推测，耐寒性的发展是由钙相关基因（CDPKs和CMLs）的表达引起的，谷胱甘肽蛋白质，和TFs(NAC，WRKY,和ERF）。我们的调查发现，三个品种在田间暴露于-9°C至-15°C的温度下会遭受冷害。因此,越冬期间蓝莓的耐寒性不仅要抵抗低温的影响，还要抵抗大风和空气中相对湿度低等复杂环境因素的影响。三个蓝莓品种的抗寒强度顺序为“Berkeley”，\'Bluecrop\',和“北国”。这些结果提供了对冷应激的反应的全面概况，它有可能被用作提高蓝莓耐寒性的项目的选择标记。
