PDF(Pubmed)
Abstract：
Doxorubicin (DOX) has been an effective antitumor agent for human liver cancer cells; however, an overdose might lead to major side effects appearing in clinical applications. In this work, we present a strategy of combining DOX and blue light (BL) irradiation for the antitumor treatment of HepG2 cells (one typical human liver cancer cell line). It is demonstrated that synergetic DOX and BL can significantly reduce cell proliferation and increase the apoptotic rate of HepG2 cells in comparison to individual DOX treatment. The additional BL irradiation is further helpful for enhancing the inhibition of cell migration and invasion. Analyses of reactive oxygen species (ROS) level and Western blotting reveal that the strategy results in more ROS accumulation, mitochondrial damage, and the upregulation of proapoptotic protein (Bcl-2) and downregulation of antiapoptotic protein (Bax). In addition to the improved therapeutic effect, the non-contact BL irradiation is greatly helpful for reducing the dosage of DOX, and subsequently reduces the side effects caused by the DOX drug. These findings offer a novel perspective for the therapeutic approach toward liver cancer with high efficiency and reduced side effects.
摘要：
多柔比星(DOX)已成为一种有效的人肝癌细胞抗肿瘤剂,然而,用药过量可能导致临床应用中出现的主要副作用。在这项工作中,我们提出了一种结合DOX和蓝光（BL）照射的策略，用于HepG2细胞（一种典型的人肝癌细胞系）的抗肿瘤治疗。结果表明，与单独的DOX处理相比，协同的DOX和BL可以显着降低细胞增殖并增加HepG2细胞的凋亡率。额外的BL照射进一步有助于增强对细胞迁移和侵袭的抑制。活性氧（ROS）水平和蛋白质印迹分析表明，该策略导致更多的ROS积累，线粒体损伤，促凋亡蛋白(Bcl-2)的上调和抗凋亡蛋白(Bax)的下调。除了改善治疗效果外，非接触式BL照射对降低DOX的用量有很大帮助，并随后减少由DOX药物引起的副作用。这些发现为高效和减少副作用的肝癌治疗方法提供了新的视角。
