PDF(Pubmed)
Abstract：
Grape seeds are rich in bioactive substances, including polyphenols, terpenoids, and phytosterols. Linseed (Linum usitatissimum L.) boasts a high concentration of polyunsaturated fatty acids (PUFAs), lignans, phytoestrogens, and soluble fibers, all contributing to its therapeutic potential. In this study, we pioneered the formulation of an oil blend (GL) combining grape seed oil (G) and golden linseed oil (GL) in equal volumes (1:1 (v/v)) and we evaluated in terms of the nutritional, physical, and chemical properties and their influence in an in vivo experimental model. We analyzed the oils by performing physical-chemical analyses, examining the oxidative stability using Rancimat; conducting thermal analyses via thermogravimetry/derivative thermogravimetry (TG/DTG) and differential scanning calorimetry (DSC), performing optical UV-vis absorption analyses; examining the fluorescence emission-excitation matrix, total carotenoids, and color, and conducting metabolic assessments in an in vivo experimental trial. The fatty acid profile presented a higher fraction of linoleic acid (C18:2) in G and GL and alpha-linolenic acid (C18:3) in L. The acidity and peroxide indices were within the recommended ranges. The TG/DTG, DSC, and Rancimat analyses revealed similar behaviors, and the optical analyses revealed color variations caused by carotenoid contents in L and GL. In the in vivo trial, G (G2: 2000 mg/kg/day) promoted lower total consumption, and the blend (GL: 2000 mg/kg/day) group exhibited less weight gain per gram of consumed food. The group with G supplementation (G2: 2000 mg/kg/day) and GL had the highest levels of HDL-c. The group with L supplementation (L2: 2000 mg/kg/day) had the lowest total cholesterol level. The L2, G1 (1000 mg/kg/day), and G2 groups exhibited the lowest MCP-1 and TNF-α values. Additionally, the lowest adipocyte areas occurred in G and GL. Our results suggest that this combination is of high quality for consumption and can influence lipid profiles, markers of inflammation, and antioxidant status.
摘要：
葡萄籽富含生物活性物质,包括多酚，萜类化合物,和植物甾醇。亚麻籽（LinumusitatissimumL.）拥有高浓度的多不饱和脂肪酸（PUFA），木脂素,植物雌激素,和可溶性纤维，都有助于其治疗潜力。在这项研究中,我们率先制定了混合葡萄籽油（G）和金色亚麻籽油（GL）等体积（1：1（v/v））的油混合物（GL）的配方，物理，和化学性质及其在体内实验模型中的影响。我们通过进行物理化学分析来分析油，使用Rancimat检查氧化稳定性；通过热重法/衍生热重法（TG/DTG）和差示扫描量热法（DSC）进行热分析，进行光学UV-vis吸收分析；检查荧光发射激发矩阵，总类胡萝卜素，和颜色,并在体内实验试验中进行代谢评估。脂肪酸谱在G和GL中的亚油酸（C18：2）和L中的α-亚麻酸（C18：3）含量较高。酸度和过氧化物指数在推荐范围内。TG/DTG,DSC,Rancimat分析揭示了类似的行为，光学分析显示L和GL中类胡萝卜素含量引起的颜色变化。在体内试验中,G（G2：2000毫克/千克/天）促进了总消费量的降低，并且共混物(GL:2000mg/kg/天)组表现出每克消耗的食物较少的体重增加。补充G（G2：2000mg/kg/天）和GL的组HDL-c水平最高。补充L的组(L2:2000mg/kg/天)具有最低的总胆固醇水平。L2，G1（1000毫克/千克/天），和G2组表现出最低的MCP-1和TNF-α值。此外,脂肪细胞最低区出现在G和GL.我们的结果表明，这种组合是高品质的消费，可以影响血脂谱，炎症的标志，和抗氧化状态。
