PDF(Pubmed)
Abstract：
Chlorich®EnergyBoost, a water extract obtained from Chlorella sorokiniana, has been proposed to enhance physical performance and provide anti-fatigue effects. This study assessed the impact of Chlorich®EnergyBoost supplementation on physical performance and its anti-fatigue properties. Twenty-four mice were allocated into four groups: (1) the control group receiving only water,;(2) the 1X group (49.2 mg/kg/day); (3) the 2X group (98.4 g/kg/day); and (4) the 5X group (246 g/kg/day). All groups were orally administered the supplements for four consecutive weeks. The evaluation included grip strength, swimming endurance, an exhaustion test, and serum biochemistry analysis. Additionally, the study examined the bioactive peptides through matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) and conducted bacterial reverse mutation and acute oral toxicity tests for safety assessment. The findings indicated that Chlorich®EnergyBoost supplementation led to a significant reduction in serum lactate levels by 14.08% to 22.54% and blood urea nitrogen levels by 12.23% to 16.76%, an increase in the lactate clearance rate by 0.28 to 0.35, an enhancement of muscle glycogen storage by 1.10 to 1.44-fold, and hepatic glycogen storage by 1.41 to 1.47-fold. These results demonstrated dose-dependent effects. MALDI-TOF analysis revealed the expression of dihydrolipoamide dehydrogenase and superoxide dismutase. Both the bacterial reverse mutation and acute oral toxicity tests showed no adverse effects.
摘要：
Chlorich®EnergyBoost,从小球藻获得的水提取物，已经提出了增强物理性能和提供抗疲劳效果。这项研究评估了Chlorich®EnergyBoost补充剂对物理性能及其抗疲劳性能的影响。将24只小鼠分为四组：（1）对照组仅接受水，（2）1X组（49.2mg/kg/天）；（3）2X组（98.4g/kg/天）；（4）5X组（246g/kg/天）。所有组均连续4周口服补充剂。评估包括握力，游泳耐力,疲劳试验,和血清生化分析。此外,该研究通过基质辅助激光解吸/电离质谱(MALDI-TOFMS)检测了生物活性肽,并进行了细菌回复突变和急性口服毒性试验,以进行安全性评估.研究结果表明，补充Chlorich®EnergyBoost导致血清乳酸水平显着降低14.08％至22.54％，血液尿素氮水平显着降低12.23％至16.76％，乳酸清除率增加0.28至0.35，肌肉糖原储存增加1.10至1.44倍，肝糖原贮量为1.41至1.47倍。这些结果证明了剂量依赖性效应。MALDI-TOF分析显示二氢硫酰胺脱氢酶和超氧化物歧化酶的表达。细菌回复突变和急性经口毒性试验均未见不良反应。
