PDF(Pubmed)
Abstract：
Temporal lobe epilepsy has various origins, involving or not involving structural changes in brain tissue. The mechanisms of epileptogenesis are associated with cell regulation and signaling disruptions expressed in varied levels of proteins. The blood plasma proteomic profiling of temporal lobe epilepsy patients (including magnetic resonance imaging (MRI)-positive and MRI-negative ones) and healthy volunteers using mass spectrometry and label-free quantification revealed a list of differently expressed proteins. Several apolipoproteins (APOA1, APOD, and APOA4), serpin protease inhibitors (SERPINA3, SERPINF1, etc.), complement components (C9, C8, and C1R), and a total of 42 proteins were found to be significantly upregulated in the temporal lobe epilepsy group. A classification analysis of these proteins according to their biological functions, as well as a review of the published sources, disclosed the predominant involvement of the processes mostly affected during epilepsy such as neuroinflammation, intracellular signaling, lipid metabolism, and oxidative stress. The presence of several proteins related to the corresponding compensatory mechanisms has been noted. After further validation, the newly identified temporal lobe epilepsy biomarker candidates may be used as epilepsy diagnostic tools, in addition to other less specific methods such as electroencephalography or MRI.
摘要：
颞叶癫痫有各种起源，涉及或不涉及脑组织的结构变化。癫痫发生的机制与细胞调节和以不同水平的蛋白质表达的信号传导中断有关。使用质谱和无标记定量对颞叶癫痫患者（包括磁共振成像（MRI）阳性和MRI阴性）和健康志愿者的血浆蛋白质组学进行分析，发现了一系列不同表达的蛋白质。几种载脂蛋白（APOA1，APOD，和APOA4），serpin蛋白酶抑制剂（SERPINA3，SERPINF1等。),补体成分（C9，C8和C1R），在颞叶癫痫组中，共有42种蛋白质被显著上调。根据这些蛋白质的生物学功能进行分类分析，以及对已发布来源的评论，披露了在癫痫期间主要受影响的过程的主要参与，如神经炎症，细胞内信号，脂质代谢，和氧化应激。已经注意到与相应的补偿机制相关的几种蛋白质的存在。经过进一步验证，新发现的颞叶癫痫候选生物标志物可用作癫痫诊断工具，除了其他不太具体的方法，如脑电图或MRI。
