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Abstract：
Rosemary has many medicinal and therapeutic properties and therefore it is important to study how to maximize the recovery of its bioactive compounds. In the present study, four different extraction techniques were used, namely stirring extraction (STE), pulsed electric field-assisted extraction (PEF), ultrasound probe-assisted extraction (UPAE), and ultrasound bath-assisted extraction (UBAE). First, some primary experiments were carried out in order to optimize each technique individually through the Plackett-Burman design. Then, each technique was applied under optimal conditions and the results were compared with each other. The optimal total polyphenol content (TPC) of STE is ~19 mg gallic acid equivalents per gram of dry weight (dw), while the antioxidant activity of the extract is 162 μmol ascorbic acid equivalents (AAEs) per gram of dw via FRAP and ~110 μmol AAE per gram of dw via DPPH. As for PEF, the optimal TPC is ~12 mg GAE/g dw, and the FRAP and DPPH values are ~102 and ~70 μmol AAE per gram of dw, respectively. When it comes to UPAE, the optimal TPC is ~16 mg GAE/g dw and the antioxidant capacity of the extract is ~128 μmol AAE/g dw through FRAP and ~98 μmol AAE/g dw through DPPH. UBAE optimal extract yielded ~17 mg GAE/g dw TPC, ~146 μmol AAE/g dw for FRAP, and ~143 μmol AAE/g dw for DPPH. The highest flavonoid content (~6.5 mg rutin equivalent/g dw) and DPPH (~143 μmol ascorbic acid equivalent/g dw) is obtained through UBAE. UPAE has been shown to be more efficient in recovering ascorbic acid (~20 mg/g dw). Additionally, the chlorophyll-to-carotenoid ratios of UPAE and UBAE were 2.98 and 2.96, respectively, indicating that the extracts had a generally positive impact on health. Considering the environmental impact of each extraction technique but also which antioxidant factor needs to be maximized, the most suitable extraction technique will be chosen.
摘要：
迷迭香具有许多药用和治疗特性，因此重要的是研究如何最大限度地恢复其生物活性化合物。在本研究中,使用了四种不同的提取技术，即搅拌萃取(STE)，脉冲电场辅助萃取（PEF），超声探头辅助提取（UPAE），超声浴辅助提取(UBAE)。首先,为了通过Plackett-Burman设计单独优化每种技术，进行了一些主要实验。然后，每种技术都在最佳条件下应用，并将结果相互比较。STE的最佳总多酚含量（TPC）为每克干重（dw）〜19mg没食子酸当量，而提取物的抗氧化活性为通过FRAP每克dw的162μmol抗坏血酸当量（AAE）和通过DPPH每克dw的〜110μmolAAE。至于PEF,最佳TPC为〜12mgGAE/gdw，FRAP和DPPH值分别为每克dw~102和~70μmolAAE，分别。说到UPAE,最佳TPC为〜16mgGAE/gdw，提取物的抗氧化能力通过FRAP为〜128μmolAAE/gdw，通过DPPH为〜98μmolAAE/gdw。UBAE最佳提取物产生约17mgGAE/gdwTPC，FRAP为~146μmolAAE/gdw，DPPH为~143μmolAAE/gdw。通过UBAE获得最高的类黄酮含量（〜6.5mg芦丁当量/gdw）和DPPH（〜143μmol抗坏血酸当量/gdw）。UPAE已被证明在回收抗坏血酸(〜20mg/gdw)方面更有效。此外,UPAE和UBAE的叶绿素与类胡萝卜素的比率分别为2.98和2.96，表明提取物对健康有普遍的积极影响。考虑到每种提取技术对环境的影响，以及需要最大化的抗氧化因子，将选择最合适的提取技术。
