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Abstract：
Porcine deltacoronavirus (PDCoV) is an emerging enteric pathogen that causes substantial economic losses in the swine industry worldwide. The PDCoV NS6 protein is an accessory protein that plays a pivotal role in the viral life cycle and immune evasion. However, the functions of NS6 and its role in PDCoV pathogenesis remain largely unknown. In this study, we prepared a monoclonal antibody (mAb) 5-A11 that specifically recognizes the PDCoV NS6 protein. The mAb 5-A11 exhibited high specificity for PDCoV, with no cross-reactivity with several major porcine pathogenic viruses. Furthermore, the epitope recognized by mAb 5-A11 was precisely mapped to residues 70EYGSIYGKDFI80 of the NS6 protein using Western blot analysis. Notably, this epitope is highly conserved among different PDCoV isolates. Substantial variations were observed when comparing this epitope with the corresponding regions in the NS6 proteins of other δ coronaviruses, suggesting potential differences in the structure, function, and antigenicity of their NS6 proteins. Our findings provide valuable tools and insights for further elucidating the functions of the NS6 protein and its role in PDCoV pathogenesis, as well as for developing diagnostic and therapeutic strategies against PDCoV infection.
摘要：
猪deltacronavirus（PDCoV）是一种新兴的肠道病原体，在全世界的养猪业中造成巨大的经济损失。PDCoVNS6蛋白是在病毒生命周期和免疫逃避中起关键作用的辅助蛋白。然而,NS6的功能及其在PDCoV发病机制中的作用尚不清楚.在这项研究中,我们制备了特异性识别PDCoVNS6蛋白的单克隆抗体（mAb）5-A11。单克隆抗体5-A11对PDCoV表现出很高的特异性，与几种主要的猪致病性病毒没有交叉反应。此外，通过Westernblot分析,mAb5-A11识别的表位被精确定位到NS6蛋白的70EYGSIYGKDFI80残基上.值得注意的是,该表位在不同的PDCoV分离株中高度保守.当将该表位与其他δ冠状病毒的NS6蛋白中的相应区域进行比较时，观察到了实质性的变化。暗示结构中的潜在差异，函数,和它们的NS6蛋白的抗原性。我们的发现为进一步阐明NS6蛋白的功能及其在PDCoV发病机制中的作用提供了有价值的工具和见解。以及用于开发针对PDCoV感染的诊断和治疗策略。
