PDF(Pubmed)
Abstract：
BACKGROUND: Mitochondria play a crucial role in adapting to fluctuating energy demands, particularly in various heart diseases. This study investigates mitochondrial morphology near intercalated discs in left ventricular (LV) heart tissues, comparing samples from patients with sinus rhythm (SR), atrial fibrillation (AF), dilated cardiomyopathy (DCM), and ischemic cardiomyopathy (ICM).
METHODS: Transmission electron microscopy was used to analyze mitochondria within 0-3.5 μm and 3.5-7 μm of intercalated discs in 9 SR, 10 AF, 9 DCM, and 8 ICM patient samples. Parameters included mean size in µm2 and elongation, count, percental mitochondrial area in the measuring frame, and a conglomeration score.
RESULTS: AF patients exhibited higher counts of small mitochondria in the LV myocardium, resembling SR. DCM and ICM groups had fewer, larger, and often hydropic mitochondria. Accumulation rates and percental mitochondrial area were similar across groups. Significant positive correlations existed between other defects/size and hydropic mitochondria and between count/area and conglomeration score, while negative correlations between count and size/other defects and between hydropic mitochondria and count could be seen as well.
CONCLUSIONS: Mitochondrial parameters in the LV myocardium of AF patients were similar to those of SR patients, while DCM and ICM displayed distinct changes, including a decrease in number, an increase in size, and compromised mitochondrial morphology. Further research is needed to fully elucidate the pathophysiological role of mitochondrial morphology in different heart diseases, providing deeper insights into potential therapeutic targets and interventions.
METHODS: Transmission electron microscopy was used to analyze mitochondria within 0-3.5 μm and 3.5-7 μm of intercalated discs in 9 SR, 10 AF, 9 DCM, and 8 ICM patient samples. Parameters included mean size in µm2 and elongation, count, percental mitochondrial area in the measuring frame, and a conglomeration score.
RESULTS: AF patients exhibited higher counts of small mitochondria in the LV myocardium, resembling SR. DCM and ICM groups had fewer, larger, and often hydropic mitochondria. Accumulation rates and percental mitochondrial area were similar across groups. Significant positive correlations existed between other defects/size and hydropic mitochondria and between count/area and conglomeration score, while negative correlations between count and size/other defects and between hydropic mitochondria and count could be seen as well.
CONCLUSIONS: Mitochondrial parameters in the LV myocardium of AF patients were similar to those of SR patients, while DCM and ICM displayed distinct changes, including a decrease in number, an increase in size, and compromised mitochondrial morphology. Further research is needed to fully elucidate the pathophysiological role of mitochondrial morphology in different heart diseases, providing deeper insights into potential therapeutic targets and interventions.
摘要：
背景：线粒体在适应波动的能量需求中起着至关重要的作用，特别是在各种心脏病中。这项研究调查了左心室（LV）心脏组织中插入椎间盘附近的线粒体形态，比较来自窦性心律（SR）患者的样本，心房颤动(AF),扩张型心肌病（DCM），和缺血性心肌病（ICM）。
方法：透射电子显微镜用于分析9SR中插入盘的0-3.5μm和3.5-7μm范围内的线粒体，10AF,9DCM，和8个ICM患者样本。参数包括以µm2为单位的平均尺寸和伸长率，计数,测量框中的线粒体百分比面积，和集团评分。
结果：AF患者在左心室心肌中表现出更高的小线粒体计数，类似于SR。DCM和ICM组较少，较大,和经常是水肿的线粒体。各组的积累率和线粒体面积百分比相似。其他缺陷/大小与水肿线粒体之间以及计数/面积与聚集评分之间存在显着正相关，而计数与大小/其他缺陷之间以及水样线粒体与计数之间也呈负相关。
结论：AF患者左心室心肌线粒体参数与SR患者相似，虽然DCM和ICM显示了明显的变化，包括数量的减少，尺寸的增加，线粒体形态受损。需要进一步的研究来充分阐明线粒体形态在不同心脏疾病中的病理生理作用。为潜在的治疗目标和干预措施提供更深入的见解。
方法：透射电子显微镜用于分析9SR中插入盘的0-3.5μm和3.5-7μm范围内的线粒体，10AF,9DCM，和8个ICM患者样本。参数包括以µm2为单位的平均尺寸和伸长率，计数,测量框中的线粒体百分比面积，和集团评分。
结果：AF患者在左心室心肌中表现出更高的小线粒体计数，类似于SR。DCM和ICM组较少，较大,和经常是水肿的线粒体。各组的积累率和线粒体面积百分比相似。其他缺陷/大小与水肿线粒体之间以及计数/面积与聚集评分之间存在显着正相关，而计数与大小/其他缺陷之间以及水样线粒体与计数之间也呈负相关。
结论：AF患者左心室心肌线粒体参数与SR患者相似，虽然DCM和ICM显示了明显的变化，包括数量的减少，尺寸的增加，线粒体形态受损。需要进一步的研究来充分阐明线粒体形态在不同心脏疾病中的病理生理作用。为潜在的治疗目标和干预措施提供更深入的见解。
