PDF(Pubmed)
Abstract：
To assess the effects of hydroxysafflor yellow A (HSYA) on ultraviolet A (UVA)-induced damage in HaCaT keratinocytes. HaCaT keratinocytes were UVA-irradiated, and the effects of HSYA on cell viability, reactive oxygen species (ROS) generation, lipid peroxidation, and messenger (m)RNA expression were measured. mRNA expressions of matrix metalloproteinase (MMP)-1, MMP-2, MMP-9, and cyclooxygenase (COX)-2 were determined by a real-time polymerase chain reaction (RT-PCR). UVA exposure led to a decrease in cell viability and an increase in ROS generation in HaCaT keratinocytes. HSYA effectively increased the viability of HaCaT keratinocytes after UVA exposure and protected them from UVA-induced oxidative stress. Moreover, HSYA inhibited expressions of MMP-1, MMP-2, MMP-9, and COX-2 by HaCaT keratinocytes with UVA-induced photodamage. Our results suggest that HSYA can act as a free radical scavenger when keratinocytes are photodamaged. HSYA has the potential to be a skin-protective ingredient against UVA-induced photodamage.
摘要：
评价羟基红花黄色素A(HSYA)对紫外线A(UVA)诱导的HaCaT角质形成细胞损伤的影响。HaCaT角质形成细胞经UVA照射，以及HSYA对细胞活力的影响，活性氧（ROS）的产生，脂质过氧化，和信使(m)RNA表达被测量。通过实时聚合酶链反应（RT-PCR）检测基质金属蛋白酶（MMP）-1，MMP-2，MMP-9和环氧合酶（COX）-2的mRNA表达。UVA暴露导致HaCaT角质形成细胞中细胞活力降低和ROS生成增加。HSYA有效增加UVA暴露后HaCaT角质形成细胞的活力，并保护它们免受UVA诱导的氧化应激。此外，HSYA抑制UVA诱导的HaCaT角质形成细胞的MMP-1，MMP-2，MMP-9和COX-2的表达。我们的结果表明，当角质形成细胞受到光损伤时，HSYA可以充当自由基清除剂。HSYA有可能成为对抗UVA诱导的光损伤的皮肤保护成分。
