PDF(Pubmed)
Abstract：
TGF-β1/Smads is a classic signaling pathway, which plays important roles in the development process of organisms. Black porgy Acanthopagrus schlegelii and red porgy Pagrus major are valuable economic fishes, and their hybrid offspring show excellent heterosis traits. Yet the molecular regulation mechanism of the heterosis traits is less clear. Here, we explored the TGF-β1/Smads pathway\'s molecular genetic information for heterosis in A. schlegelii ♂ × P. major ♀ (AP) and A. schlegelii ♀ × P. major ♂ (PA) in terms of growth and development. The mRNA expression levels of TGF-β1, TβR-I, TβR-II, and Smad2 genes in different developmental stages of A. schlegelii were detected. Furthermore, the expression levels of TGF-β1, TβR-I, TβR-II, and Smad2 genes in different tissues of adult (mRNA level) and larva (mRNA and protein level) of A. schlegelii, P. major, and their hybrids were determined by both real-time quantitative PCR and Western blot techniques. The results indicated the ubiquitous expression of these genes in all developmental stages of A. schlegelii and in all tested tissues of A. schlegelii, P. major, and its hybrids. Among them, the mRNA of TGF-β1, TβR-I, and TβR-II genes is highly expressed in the liver, gill, kidney, and muscle of black porgy, red porgy, and their hybrid offspring. There are significant changes in gene and protein expression levels in hybrid offspring, which indirectly reflect hybrid advantage. In addition, there was no correlation between protein and mRNA expression levels of Smad2 protein. The results provide novel data for the differential expression of growth and development genes between the reciprocal hybridization generation of black porgy and red porgy and its parents, which is conducive to further explaining the molecular regulation mechanism of heterosis in the growth and development of hybrid porgy.
摘要：
TGF-β1/Smads是一个经典的信号通路,在生物体的发育过程中起着重要的作用。黑色的刺五加草和红色的刺五加草主要是有价值的经济鱼类，它们的杂交后代表现出优异的杂种优势。然而杂种优势性状的分子调控机制尚不清楚。这里,我们探索了TGF-β1/Smads途径中杂种优势的分子遗传信息。从生长和发育角度来看，主要是P。TGF-β1、TβR-I的mRNA表达水平,TβR-II,在不同发育阶段的Schlegelii中检测到Smad2基因。此外，TGF-β1，TβR-I的表达水平，TβR-II,和Smad2基因在成虫（mRNA水平）和幼虫（mRNA和蛋白质水平）的不同组织中，P.少校，并通过实时定量PCR和Western印迹技术确定其杂种。结果表明，这些基因在A.schlegelii的所有发育阶段和A.schlegelii的所有测试组织中普遍存在，P.少校，和它的混血儿。其中,TGF-β1，TβR-I，TβR-II基因在肝脏中高表达，吉尔,肾,和黑猪的肌肉，红色的波吉,和他们的杂交后代。杂交后代的基因和蛋白质表达水平有显著变化,间接反映了混合优势。此外,Smad2蛋白mRNA表达水平与蛋白表达水平无相关性。研究结果为黑鸽和红鸽相互杂交世代及其亲本之间生长发育基因的差异表达提供了新的数据,有利于进一步解释杂种优势在杂种优势生长发育中的分子调控机制。
