Abstract:
Vitis vinifera L. is a natural source of bioactive compounds that is already used for cosmeceutical and nutraceutical approaches. However, their phytochemical and antioxidant properties, although studied, have not been fully explored. We aimed to characterize V. vinifera L. cv. Falanghina seed extracts in different polarity solvents (hexane, ethyl acetate, ethanol, and a mixture of acetone-water) for their phytochemical contents, including the total phenolic compound content (TPC), free radical scavenging capacities, and antioxidant ability on HepG2 cells. We directly profiled the functional quality of V. vinifera seed extracts against H2O2-induced oxidative stress in HepG2 cells, focusing on mitochondrial functions. The content of bioactive compounds was characterized by LC-MS. To assess the cytocompatibility of the extracts, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was conducted. Results showed that extraction with ethyl acetate (18.12 mg GAE·g-1) and ethanol solvents (18.07 mg GAE·g-1), through Soxhlet, and with an acetone-water mixture (14.17 mg GAE·g-1), through maceration, yielded extracts rich in (poly)phenols, with good scavenging and antioxidant activity (98.32 I% for ethanol solvents and 96.31 I% for acetone-water mixture). The antioxidant effect of polyphenols is at least partially due to their capacity to maintain mitochondrial biogenesis and mitophagy, which elevates mitochondrial efficiency, resulting in diminished ROS production, hence re-establishing the mitochondrial quality control. These findings highlight the valorization of Vitis by-products to improve food functional characteristics.
摘要:
葡萄是生物活性化合物的天然来源,已用于化妆品和营养方法。然而,它们的植物化学和抗氧化特性,虽然研究过,没有得到充分的探索。我们的目的是表征V.viniferaL.cv。Falanghina种子提取物在不同极性溶剂(己烷,乙酸乙酯,乙醇,和丙酮-水的混合物)的植物化学物质含量,包括总酚类化合物含量(TPC),自由基清除能力,和对HepG2细胞的抗氧化能力。我们直接分析了V.vinifera种子提取物对H2O2诱导的HepG2细胞氧化应激的功能质量,专注于线粒体功能。通过LC-MS表征生物活性化合物的含量。为了评估提取物的细胞相容性,进行3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物(MTT)测定。结果表明,用乙酸乙酯(18.12mgGAE·g-1)和乙醇溶剂(18.07mgGAE·g-1)提取,通过索格利特,并用丙酮-水混合物(14.17mgGAE·g-1),通过浸渍,产生富含(多)酚的提取物,具有良好的清除和抗氧化活性(乙醇溶剂为98.32I%,丙酮-水混合物为96.31I%)。多酚的抗氧化作用至少部分是由于它们维持线粒体生物发生和线粒体自噬的能力,这提高了线粒体的效率,导致ROS产生减少,从而重新建立线粒体质量控制。这些发现强调了葡萄副产品的价值化以改善食品功能特性。