PDF(Pubmed)
Abstract：
The functional investigation of proteins holds immense significance in unraveling physiological and pathological mechanisms of organisms as well as advancing the development of novel pharmaceuticals in biomedicine. However, the study of cellular protein function using conventional genetic manipulation methods may yield unpredictable outcomes and erroneous conclusions. Therefore, precise modulation of protein activity within cells holds immense significance in the realm of biomedical research. Chromophore-assisted light inactivation (CALI) is a technique that labels photosensitizers onto target proteins and induces the production of reactive oxygen species through light control to achieve precise inactivation of target proteins. Based on the type and characteristics of photosensitizers, different excitation light sources and labeling methods are selected. For instance, KillerRed forms a fusion protein with the target protein through genetic engineering for labeling and inactivates the target protein via light activation. CALI is presently predominantly employed in diverse biomedical domains encompassing investigations into protein functionality and interaction, intercellular signal transduction research, as well as cancer exploration and therapy. With the continuous advancement of CALI technology, it is anticipated to emerge as a formidable instrument in the realm of life sciences, yielding more captivating outcomes for fundamental life sciences and precise disease diagnosis and treatment.
摘要：
蛋白质的功能研究对于揭示生物体的生理和病理机制以及推进生物医学中新型药物的开发具有巨大的意义。然而,使用常规遗传操作方法研究细胞蛋白质功能可能会产生不可预测的结果和错误的结论。因此,细胞内蛋白质活性的精确调节在生物医学研究领域具有巨大的意义。生色团辅助光失活(CALI)是一种将光敏剂标记到靶蛋白上并通过光控制诱导活性氧的产生以实现靶蛋白的精确失活的技术。根据光敏剂的种类和特点，选择不同的激发光源和标记方法。例如,KillerRed通过基因工程与靶蛋白形成融合蛋白，用于标记并通过光活化使靶蛋白失活。CALI目前主要用于各种生物医学领域，包括对蛋白质功能和相互作用的研究。细胞间信号转导研究,以及癌症探索和治疗。随着CALI技术的不断进步,它有望成为生命科学领域的强大工具，为基础生命科学和精确的疾病诊断和治疗产生更迷人的结果。
