PDF(Pubmed)
Abstract：
OBJECTIVE: Resveratrol is a natural polyphenolic compound with biological activities such as anti-inflammation and antioxidation. Its anti-fibrotic effect has been experimentally demonstrated in the pancreas and liver. This study aims to determine the anti-proliferative effect of resveratrol on fibroblasts obtained from hyperplastic gingival tissues from a patient diagnosed with Juvenile Hyaline Fibromatosis (JHF).
METHODS: Primary gingival fibroblast cell lines were obtained from gingival growth tissues by the gingivectomy of a patient with JHF. Gingival fibroblasts were treated with or without 3 different doses of resveratrol (50, 100, 200 µM). Cytotoxicity and cell proliferation were evaluated after 24, 48, and 72 h. Collagen, TGF, and CTGF were analyzed by ELISA in the 48-hour supernatants.
RESULTS: All three doses of resveratrol suppressed the proliferation of JHF gingival fibroblasts at 24 and 48 h without showing any cytotoxic effect compared to the control group (p < 0.0001). At 72 h, 100 and 200 µM resveratrol showed significantly less proliferation (p < 0.0001), less collagen, CTGF, and TGF- β (p < 0.001) than the control group.
CONCLUSIONS: Resveratrol had a profound anti-proliferative effect on gingival fibroblasts obtained from gingival enlargements with JHF, suggesting that it can be used as a therapeutic to prevent excessive cell growth by suppressing collagen, CTGF, and TGF- β synthesis in the pathogenesis of hyperplasia.
METHODS: Primary gingival fibroblast cell lines were obtained from gingival growth tissues by the gingivectomy of a patient with JHF. Gingival fibroblasts were treated with or without 3 different doses of resveratrol (50, 100, 200 µM). Cytotoxicity and cell proliferation were evaluated after 24, 48, and 72 h. Collagen, TGF, and CTGF were analyzed by ELISA in the 48-hour supernatants.
RESULTS: All three doses of resveratrol suppressed the proliferation of JHF gingival fibroblasts at 24 and 48 h without showing any cytotoxic effect compared to the control group (p < 0.0001). At 72 h, 100 and 200 µM resveratrol showed significantly less proliferation (p < 0.0001), less collagen, CTGF, and TGF- β (p < 0.001) than the control group.
CONCLUSIONS: Resveratrol had a profound anti-proliferative effect on gingival fibroblasts obtained from gingival enlargements with JHF, suggesting that it can be used as a therapeutic to prevent excessive cell growth by suppressing collagen, CTGF, and TGF- β synthesis in the pathogenesis of hyperplasia.
摘要：
目的：白藜芦醇是一种具有抗炎、抗氧化等生物活性的天然多酚类化合物。其抗纤维化作用已在胰腺和肝脏中得到实验证明。这项研究旨在确定白藜芦醇对成纤维细胞的抗增殖作用，这些成纤维细胞来自诊断为幼年透明纤维瘤病（JHF）的患者的增生性牙龈组织。
方法：通过JHF患者的牙龈切除术从牙龈生长组织中获得原代牙龈成纤维细胞系。用或不用3种不同剂量的白藜芦醇（50、100、200μM）处理牙龈成纤维细胞。在24、48和72小时后评估细胞毒性和细胞增殖。胶原蛋白，TGF,和CTGF在48小时上清液中通过ELISA分析。
结果：与对照组相比，所有三种剂量的白藜芦醇在24和48小时均抑制了JHF牙龈成纤维细胞的增殖，而没有表现出任何细胞毒性作用（p<0.0001）。在72小时，100和200μM白藜芦醇显示出明显更少的增殖（p<0.0001），更少的胶原蛋白，CTGF,TGF-β(p<0.001)高于对照组。
结论：白藜芦醇对从JHF牙龈扩大获得的牙龈成纤维细胞具有深远的抗增殖作用，这表明它可以用作通过抑制胶原蛋白来防止细胞过度生长的治疗剂，CTGF,TGF-β的合成与增生的发病机制有关。
方法：通过JHF患者的牙龈切除术从牙龈生长组织中获得原代牙龈成纤维细胞系。用或不用3种不同剂量的白藜芦醇（50、100、200μM）处理牙龈成纤维细胞。在24、48和72小时后评估细胞毒性和细胞增殖。胶原蛋白，TGF,和CTGF在48小时上清液中通过ELISA分析。
结果：与对照组相比，所有三种剂量的白藜芦醇在24和48小时均抑制了JHF牙龈成纤维细胞的增殖，而没有表现出任何细胞毒性作用（p<0.0001）。在72小时，100和200μM白藜芦醇显示出明显更少的增殖（p<0.0001），更少的胶原蛋白，CTGF,TGF-β(p<0.001)高于对照组。
结论：白藜芦醇对从JHF牙龈扩大获得的牙龈成纤维细胞具有深远的抗增殖作用，这表明它可以用作通过抑制胶原蛋白来防止细胞过度生长的治疗剂，CTGF,TGF-β的合成与增生的发病机制有关。
