Abstract：
OBJECTIVE: Cancer remains a major global health challenge, with an estimated 10 million cancer-related deaths in 2020, hindering efforts to extend life expectancy. Cisplatin, an effective platinum-based chemotherapeutic agent used against various malignancies, has numerous side effects. Ganoderma lucidum is a traditional Chinese medicine with extensive historical use and proven biological activity. This study investigated the effects of G. lucidum on cisplatin-induced nephrotoxicity and gastrointestinal toxicity.
METHODS: RAW264.7 cells were treated with cisplatin, G. lucidum, or both. Cytotoxicity and antioxidant capacity were measured. Slc:ICR (ICR) mice were treated with cisplatin, G. lucidum, or both. The survival rate and physiological data were measured.
RESULTS: G. lucidum suppressed cisplatin-induced cytotoxicity and apoptosis in RAW264.7 cells. G. lucidum suppressed cisplatin-induced nephrotoxicity and gastrointestinal toxicity via its antioxidant effects in ICR mice.
CONCLUSIONS: The suppressive mechanism of G. lucidum may be mediated via its antioxidant effects. These findings indicate its potential to reduce the side effects of cisplatin.
METHODS: RAW264.7 cells were treated with cisplatin, G. lucidum, or both. Cytotoxicity and antioxidant capacity were measured. Slc:ICR (ICR) mice were treated with cisplatin, G. lucidum, or both. The survival rate and physiological data were measured.
RESULTS: G. lucidum suppressed cisplatin-induced cytotoxicity and apoptosis in RAW264.7 cells. G. lucidum suppressed cisplatin-induced nephrotoxicity and gastrointestinal toxicity via its antioxidant effects in ICR mice.
CONCLUSIONS: The suppressive mechanism of G. lucidum may be mediated via its antioxidant effects. These findings indicate its potential to reduce the side effects of cisplatin.
摘要：
目的：癌症仍然是一个重大的全球卫生挑战，到2020年，估计有1000万人死于癌症，这阻碍了延长预期寿命的努力。顺铂,一种有效的铂类化疗药物，用于治疗各种恶性肿瘤，有许多副作用。灵芝是一种具有广泛历史用途和成熟生物活性的传统中药。本研究调查了灵芝对顺铂诱导的肾毒性和胃肠道毒性的影响。
方法：RAW264.7细胞用顺铂处理,G.lucidum,或者两者兼而有之。测量细胞毒性和抗氧化能力。Slc：ICR（ICR）小鼠用顺铂治疗，G.lucidum,或者两者兼而有之。测量存活率和生理数据。
结果：G.灵光能抑制顺铂诱导的RAW264.7细胞的细胞毒性和凋亡。灵芝通过其在ICR小鼠中的抗氧化作用抑制顺铂诱导的肾毒性和胃肠道毒性。
结论：灵芝的抑制机制可能是通过其抗氧化作用介导的。这些发现表明其降低顺铂副作用的潜力。
方法：RAW264.7细胞用顺铂处理,G.lucidum,或者两者兼而有之。测量细胞毒性和抗氧化能力。Slc：ICR（ICR）小鼠用顺铂治疗，G.lucidum,或者两者兼而有之。测量存活率和生理数据。
结果：G.灵光能抑制顺铂诱导的RAW264.7细胞的细胞毒性和凋亡。灵芝通过其在ICR小鼠中的抗氧化作用抑制顺铂诱导的肾毒性和胃肠道毒性。
结论：灵芝的抑制机制可能是通过其抗氧化作用介导的。这些发现表明其降低顺铂副作用的潜力。
