Abstract:
The promoting effects of collagen and its derivatives on bone health have been uncovered. However, the structure and effects of type II collagen peptides from squid cartilage (SCIIP) on osteoarthritis still need to be clarified. In this study, SCIIP was prepared from squid throat cartilage with pretreatment by 0.2 mol/L NaOH at a liquid-solid ratio of 10:1 for 18 h and hydrolyzation using alkaline protease and flavourzyme at 50 °C for 4 h. The structure of SCIIP was characterized as a molecular weight lower than 5 kDa (accounting for 87.7 %), a high glycine level of 35.0 %, typical FTIR and CD features of collagen peptides, and a repetitive sequence of Gly-X-Y. GP(Hyp)GPD and GPAGP(Hyp)GD were separated and identified from SCIIP, and their binding energies with TLR4/MD-2 were - 8.4 and - 8.0 kcal/mol, respectively. SCIIP effectively inhibited NO production in RAW264.7 macrophages and alleviated osteoarthritis in rats through the TLR4/NF-κB pathway. Therefore, SCIIP exhibited the potential for application as an anti-osteoarthritis supplement.
摘要:
胶原蛋白及其衍生物对骨骼健康的促进作用已被发现。然而,鱿鱼软骨II型胶原肽(SCIIP)的结构和对骨关节炎的作用仍有待阐明。在这项研究中,SCIIP是由鱿鱼喉软骨用0.2mol/LNaOH以10:1的液固比预处理18h,并使用碱性蛋白酶和风味酶在50°C下水解4h制备的。SCIIP的结构表征为分子量低于5kDa(占87.7%),高甘氨酸水平为35.0%,胶原蛋白肽的典型FTIR和CD特征,和Gly-X-Y的重复序列。将GP(Hyp)GPD和GPAGP(Hyp)GD与SCIIP分离,它们与TLR4/MD-2的结合能分别为-8.4和-8.0kcal/mol,分别。SCIIP通过TLR4/NF-κB通路有效抑制RAW264.7巨噬细胞NO的产生,减轻大鼠骨关节炎。因此,SCIIP显示了作为抗骨关节炎补充剂的应用潜力。