Abstract：
Phages are a class of viruses that specifically infect host bacteria. Compared to other recognition elements, phages offer several advantages such as high specificity, easy to obtain and good environmental tolerance, etc. These advantages underscore the potential of phages as recognition elements in the construction of biosensors. Therefore, the phage-based biosensors are currently garnering widespread attention for detecting pathogens in recent years. However, the test performance such as detection limit, sensitivity and stability of exicting phage-based biosensors require enhancement. In the design of sensors, the selection of various materials and construction methods significantly influences the test performance of the sensor, and employing appropriate signal amplification strategies and construction methods to devise biosensors based on different principles is an effective strategy to enhance sensor performance. The manuscript primarily focuses on the signal amplification strategies and construction methods employed in phage-based biosensors recent ten years, and summarizes the advantages and disadvantages of different signal amplification strategies and construction methods. Meanwhile, the manuscript discusses the relationship between sensor performance and various materials and construction methods, and reviews the application progress of phage-based electrochemical biosensors in the detection of foodborne bacteria. Furthermore, the manuscript points out the present limitations and the future research direction for the field of phage-based biosensors, so as to provide the reference for developing high-performance phage-based biosensors.
摘要：
噬菌体是一类特异性感染宿主细菌的病毒。与其他识别元素相比，噬菌体提供了几个优点，如高特异性，易于获得和良好的环境耐受性，等。这些优点强调了噬菌体作为生物传感器构建中的识别元件的潜力。因此,近年来，基于噬菌体的生物传感器在检测病原体方面受到了广泛的关注。然而,检测极限等测试性能，需要增强基于噬菌体的生物传感器的灵敏度和稳定性。在传感器的设计中,各种材料和构造方法的选择显著影响传感器的测试性能，采用适当的信号放大策略和构造方法设计基于不同原理的生物传感器是提高传感器性能的有效策略。该手稿主要集中在最近十年的基于噬菌体的生物传感器中采用的信号放大策略和构建方法。并总结了不同信号放大策略和构造方法的优缺点。同时,该手稿讨论了传感器性能与各种材料和构造方法之间的关系，并综述了基于噬菌体的电化学生物传感器在食源性细菌检测中的应用进展。此外，指出了目前噬菌体生物传感器领域的局限性和未来的研究方向,从而为开发高性能的基于噬菌体的生物传感器提供参考。
