Abstract：
To explore the feasibility and safety of biomaterials for posterior scleral reinforcement (PSR) in rabbits. Decellularization and genipin crosslink were applied to the fresh bovine pericardium and porcine endocranium, and then mechanical properties, suture retention strength, and stability were tested. PSR operation was performed on 24 rabbit eyes using treated biological materials. Ophthalmic examination was performed regularly before and after PSR operation (1 week, 1 month, 3 months, 6 months). To evaluate the effectiveness, A ultrasound, diopter, and optical coherence tomography were conducted. General condition, fundus photograph, and pathological examination were recorded to evaluate the safety. Compared with genipin crosslinked bovine pericardium (Gen-BP) (21.29 ± 13.29 Mpa), genipin crosslinked porcine endocranium (Gen-PE) (34.85 ± 3.67 Mpa,P< 0.01) showed a closer elastic modulus to that of genipin crosslinked human sclera. There were no complications or toxic reactions directly related to the materials. Capillary hyperplasia, inflammatory cell infiltration, and collagen fiber deposition were observed, and the content of type I collagen fibers increased after PSR. Overall, the choroidal thickness of treated eyes was significantly thickened at different time points after PSR, which were 96.84 ± 21.08 μm, 96.72 ± 22.00 μm, 90.90 ± 16.57 μm, 97.28 ± 14.74 μm, respectively. The Gen-PE group showed changes that were almost consistent with the overall data. Gen-BP and Gen-PE are safe biological materials for PSR. The Gen-PE group demonstrated more significant advantages over the Gen-BP group in terms of material properties.
摘要：
目的：＆＃xD;探讨生物材料用于兔后巩膜加固（PSR）的可行性和安全性。＆＃xD;方法：＆＃xD;将脱细胞和京尼平交联应用于新鲜的牛心包膜和猪内生膜，然后是机械性能，缝线保留强度,和稳定性进行了测试。使用处理过的生物材料对24只兔眼进行PSR手术。PSR手术前后定期进行眼科检查（1周，1个月,3个月,6个月）。为了评估有效性，超声波，屈光度,进行光学相干断层扫描(OCT)。一般情况,眼底照片（FP），并记录病理检查以评价安全性。＆＃xD;结果：＆＃xD;与京尼平交联的牛心包（Gen-BP）（21.29±13.29Mpa）相比，京尼平交联猪内生瘤(Gen-PE)(34.85±3.67Mpa,P<0.01）显示出与京尼平交联的人巩膜更接近的弹性模量。无与材料直接相关的并发症或毒性反应。毛细血管增生,炎性细胞浸润，并观察到胶原纤维沉积，PSR后Ⅰ型胶原纤维含量增加。总的来说,治疗后不同时间点的脉络膜厚度明显增厚，为96.84±21.08μm，96.72±22.00μm,90.90±16.57μm,97.28±14.74μm,分别。Gen-PE组显示出与总体数据几乎一致的变化。＆＃xD;结论：＆＃xD;Gen-BP和Gen-PE是PSR的安全生物材料。在材料性能方面，Gen-PE组表现出比Gen-BP组更明显的优势。
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