Abstract：
Bacterial infections pose a substantial threat to human health, particularly with the emergence of antibiotic-resistant strains. Therefore, it is essential to develop novel approaches for the efficient treatment of bacterial diseases. This study presents a therapeutic approach involving BBR@MMT nanosheets (NSs), wherein montmorillonite (MMT) was loaded with berberine (BBR) through an ion intercalation reaction to sterilize and promote wound healing. BBR@MMT exhibits nano-enzymatic-like catalytic activity, is easy to synthesize, and requires low reaction conditions. This nanocomplex showed photodynamic properties and superoxide dismutase (SOD) activity. The in vitro experiments indicated that BBR@MMT was able to effectively inhibit the growth of Gram-positive bacteria (S. aureus) and Gram-negative bacteria (E. coli) through the production of ROS when exposed to white light. Meanwhile, BBR@MMT inhibited the secretion of pro-inflammatory factors and scavenged free radicals via its SOD-like activity. In vivo results showed that BBR@MMT NSs were capable of effectively promoting the wound-healing process in infected mice under white light irradiation. Hence, it can be concluded that photodynamic therapy based on BBR@MMT NSs with nano-enzymatic activity has the potential to be used in treating infections and tissue repair associated with drug-resistant microorganisms.
摘要：
细菌感染对人类健康构成重大威胁,特别是抗生素耐药菌株的出现。因此,开发有效治疗细菌性疾病的新方法至关重要。本研究提出了一种涉及BBR@MMT纳米片(NS)的治疗方法，其中蒙脱石(MMT)通过离子嵌入反应加载小檗碱(BBR)以灭菌并促进伤口愈合。BBR@MMT表现出类似纳米酶的催化活性，很容易合成，并且需要低反应条件。该纳米复合物具有光动力特性和超氧化物歧化酶（SOD）活性。体外实验表明BBR@MMT能够有效抑制革兰氏阳性菌的生长(S.金黄色葡萄球菌）和革兰氏阴性菌（E.大肠杆菌)通过暴露于白光时产生ROS。同时,BBR@MMT通过其SOD样活性抑制促炎因子的分泌和清除自由基。体内实验结果表明，BBR@MMTNS能够有效促进白光照射下感染小鼠的伤口愈合过程。因此，可以得出结论，基于具有纳米酶活性的BBR@MMTNS的光动力疗法具有用于治疗与耐药微生物相关的感染和组织修复的潜力。
