PDF(Pubmed)
Abstract：
Dopamine (DA), ascorbic acid (AA), and uric acid (UA) are crucial neurochemicals, and their abnormal levels are involved in various neurological disorders. While electrodes for their detection have been developed, achieving the sensitivity required for in vivo applications remains a challenge. In this study, we proposed a synthetic Au24Cd nanoenzyme (ACNE) that significantly enhanced the electrochemical performance of metal electrodes. ACNE-modified electrodes demonstrated a remarkable 10-fold reduction in impedance compared to silver microelectrodes. Furthermore, we validated their excellent electrocatalytic activity and sensitivity using five electrochemical detection methods, including cyclic voltammetry, differential pulse voltammetry, square-wave pulse voltammetry, normal pulse voltammetry, and linear scanning voltammetry. Importantly, the stability of gold microelectrodes (Au MEs) modified with ACNEs was significantly improved, exhibiting a 30-fold enhancement compared to Au MEs. This improved performance suggests that ACNE functionalization holds great promise for developing micro-biosensors with enhanced sensitivity and stability for detecting small molecules.
摘要：
多巴胺(DA),抗坏血酸(AA),尿酸（UA）是至关重要的神经化学物质，它们的异常水平与各种神经系统疾病有关。虽然已经开发了用于检测它们的电极，实现体内应用所需的灵敏度仍然是一个挑战。在这项研究中,我们提出了一种合成Au24Cd纳米酶（ACNE），显着增强金属电极的电化学性能。与银微电极相比，ACNE修饰的电极的阻抗显着降低了10倍。此外，我们使用五种电化学检测方法验证了它们优异的电催化活性和灵敏度，包括循环伏安法，差分脉冲伏安法,方波脉冲伏安法,正常脉冲伏安法,和线性扫描伏安法。重要的是,用ACNE修饰的金微电极（AuMEs）的稳定性得到了显着改善，与AuME相比，表现出30倍的增强。这种改进的性能表明ACNE功能化对于开发具有增强的灵敏度和稳定性的用于检测小分子的微生物传感器具有巨大的前景。
