PDF(Pubmed)
Abstract：
N6-methyladenosine (m6A), a well-characterized RNA modification, is involved in regulating multiple biological processes; however, genome-wide identification and functional characterization of the m6A modification in legume plants, including soybean (Glycine max (L.) Merr.), remains lacking. In this study, we utilized bioinformatics tools to perform comprehensive analyses of molecular writer candidates associated with the RNA m6A modification in soybean, characterizing their conserved domains, motifs, gene structures, promoters, and spatial expression patterns. Thirteen m6A writer complex genes in soybean were identified, which were assigned to four families: MT-A70, WTAP, VIR, and HAKAI. It also can be identified that multiple cis elements in the promoters of these genes, which were classified into five distinct groups, including elements responsive to light, phytohormone regulation, environmental stress, development, and others, suggesting that these genes may modulate various cellular and physiological processes in plants. Importantly, the enzymatic activities of two identified m6A writers, GmMTA1 and GmMTA2, were confirmed in vitro. Furthermore, we analyzed the expression patterns of the GmMTAs and GmMTBs under different abiotic stresses, revealing their potential involvement in stress tolerance, especially in the response to alkalinity or darkness. Overexpressing GmMTA2 and GmMTB1 in soybean altered the tolerance of the plants to alkalinity and long-term darkness, further confirming their effect on the stress response. Collectively, our findings identified the RNA m6A writer candidates in leguminous plants and highlighted the potential roles of GmMTAs and GmMTBs in the response to abiotic stress in soybean.
摘要：
N6-甲基腺苷（m6A），特征良好的RNA修饰，参与调节多个生物过程；然而，豆科植物中m6A修饰的全基因组鉴定和功能表征，包括大豆(甘氨酸max(L.）合并。),仍然缺乏。在这项研究中,我们利用生物信息学工具对大豆中与RNAm6A修饰相关的分子作者进行了综合分析，表征它们的保守域，图案，基因结构，promotors,和空间表达模式。在大豆中鉴定出13个m6Awriter复杂基因，分配给四个家庭：MT-A70，WTAP，VIR,和HAKAI。还可以确定这些基因的启动子中的多个顺式元件，分为五个不同的组，包括对光有反应的元素，植物激素调节，环境压力，发展,和其他人,表明这些基因可以调节植物的各种细胞和生理过程。重要的是,两名鉴定的M6A作者的酶活性，在体外证实了GmMTA1和GmMTA2。此外，我们分析了GmMTAs和GmMTBs在不同非生物胁迫下的表达模式，揭示了他们在压力耐受性方面的潜在参与，尤其是对碱性或黑暗的反应。大豆中GmMTA2和GmMTB1的过表达改变了植株对碱性和长期黑暗的耐受性,进一步证实了它们对应激反应的影响。总的来说，我们的发现确定了豆科植物中的RNAm6A作家候选人，并强调了GmMTAs和GmMTBs在大豆对非生物胁迫的反应中的潜在作用。
