Abstract：
2-O-β-D-Glucopyranosyl-L-ascorbic acid (AA-2βG) from Lycium barbarum fruits has diverse bioactivities, yet its absorption and digestion are poorly understood. Therefore, the in vivo absorption of AA-2βG in rats was investigated in the present study. After oral administration to SD rats, AA-2βG was absorbed intact, reaching a peak plasma concentration of 472.32 ± 296.64 nM at 90 min, with fecal excretion peaking at 4-8 h and decreasing rapidly by 12-24 h, indicating a prolonged intestinal presence. Furthermore, the digestibility under simulated gastrointestinal conditions and the impact on the gut flora through in vitro fermentation of AA-2βG were investigated. The results reveal that AA-2βG resisted in in vitro simulated digestion, indicating potential interactions with the gut microbiota. The results of in vitro fermentation showed that AA-2βG regulated the composition of the gut microbiota by promoting Oscillospiraceae, Faecalibacterium, Limosilactobacillus, and Fusicatenibacter, while inhibiting Enterococcus, Phocaeicola, Bacteroides, and Streptococcus. Furthermore, at the species level, AA-2βG promoted the growth of Limosilactobacillus mucosae and Faecalibacterium prausnitzii, and inhibited the growth of Enterococcus. F. prausnitzii is a major producer of n-butyric acid, and the results of short-chain fatty acids also demonstrated a significant promotion of n-butyric acid. Therefore, the study on the absorption, excretion, and regulatory effects of AA-2βG on the gut microbiota supported its potential development as a functional food additive to enhance intestinal health and prevent diseases.
摘要：
枸杞果实中的2-O-β-D-吡喃葡萄糖基-L-抗坏血酸（AA-2βG）具有多种生物活性，然而，它的吸收和消化却知之甚少。因此,本研究研究了大鼠体内AA-2βG的吸收。口服给SD大鼠后，AA-2βG完整吸收,在90分钟时达到472.32±296.64nM的峰值血浆浓度，粪便排泄在4-8小时达到峰值，并在12-24小时迅速减少，表明长时间的肠道存在。此外，研究了体外发酵AA-2βG在模拟胃肠道条件下的消化率以及对肠道菌群的影响。结果表明，AA-2βG在体外模拟消化中具有抗性，表明与肠道微生物群的潜在相互作用。体外发酵结果表明,AA-2βG通过促进Oscillospiraceae,粪杆菌,Limosilactacillus,和镰刀菌,在抑制肠球菌的同时，Phocaeicola,拟杆菌,和链球菌。此外，在物种层面，AA-2βG促进粘膜Limosilactobacillus和prausnitzii的生长，抑制肠球菌的生长。F.prausnitzii是正丁酸的主要生产商，短链脂肪酸的结果也证明了正丁酸的显着促进作用。因此,对吸收的研究，排泄,AA-2βG对肠道微生物群的调节作用支持其作为功能性食品添加剂的潜在发展，以增强肠道健康和预防疾病。
