Abstract：
The presence of arsenic in the environment is a public health problem. Groundwater of certain regions of Argentina contains arsenic of natural origin in concentrations that exceed the guide level recommended by World Health Organization (WHO, 10 µg/L). Pathologies derived from chronic arsenic consumption justify the planning of human biomonitoring. Hence, the aim of this study was to evaluate oxidative damage and genotoxicity and its relationship with nutritional variables in populations exposed to arsenic through drinking water in Santa Fe province, Argentina. A total of 322 participants were analyzed for arsenic in urine together with biomarkers of genotoxicity (Comet assay in blood and frequency of Micronuclei and other Nuclear Abnormalities in exfoliated buccal cells) and oxidative stress (modified Comet assay with Endonuclease III, Lipid peroxidation and antioxidant enzyme activity), as well as nutritional and biochemical variables. Results showed that 45 % of participants excreted arsenic in the urine. Consumption of water with arsenic, whether currently or previously, was associated with statistically significant increase of oxidative DNA damage and lipid peroxidation. MN in exfoliated buccal cells serve as an early biomarker of genotoxicity and showed significant differences in the current exposed group. Biochemical results indicate dyslipidemias potentially linked to dietary choices, and insufficient intake of fruits and vegetables rich in antioxidants, was also noted. This study advocates risk communication to the population, educators, and health authorities, emphasizing the need for preventive health strategies and improved food education.
摘要：
环境中砷的存在是一个公共卫生问题。阿根廷某些地区的地下水含有天然来源的砷，其浓度超过了世界卫生组织（WHO，10µg/L)。慢性砷消耗引起的病理证明了人类生物监测的计划。因此，这项研究的目的是评估圣达菲省通过饮用水暴露于砷的人群的氧化损伤和遗传毒性及其与营养变量的关系，阿根廷。总共分析了322名参与者的尿液中砷以及遗传毒性的生物标志物（血液中的彗星测定以及脱落的口腔细胞中的微核和其他核异常的频率）和氧化应激（使用内切核酸酶III进行的改良彗星测定，脂质过氧化和抗氧化酶活性），以及营养和生化变量。结果显示，45％的参与者在尿液中排泄砷。消耗含砷的水，无论是当前还是以前，与DNA氧化损伤和脂质过氧化的统计学显着增加相关。面颊脱落细胞中的MN可作为遗传毒性的早期生物标志物，并在当前暴露组中显示出显着差异。生化结果表明血脂异常可能与饮食选择有关，富含抗氧化剂的水果和蔬菜摄入不足，也注意到了。这项研究提倡与人群进行风险沟通，教育工作者,和卫生当局，强调预防性健康战略和改善食品教育的必要性。
