Abstract：
OBJECTIVE: The goal of the present study was to examine the repeated dose 28-day oral toxicity of curcumin, anthocyanins, and sodium nitrite in Wistar rats.
METHODS: For this purpose, forty-eight male Wistar rats were randomly divided into 8 groups (n = 6 each), encompassing untreated controls and experimental groups treated with curcumin, anthocyanins, and sodium nitrite. Three rats from each group were sacrificed by cervical dislocation under di-ethyl ether anesthesia after 2 and 4 weeks of therapy, respectively. Blood samples were collected for serum chemistry. All of the animals\' livers, hearts, and kidneys were removed and sent for histopathological examination.
RESULTS: After two weeks of inquiry, certain groups displayed higher hematological values, while others had lower values compared to the control group. AST, CK, and LDH enzyme activity were higher in groups 2-8, but urea concentrations were higher in groups 6 and 8. After four weeks, the Hb, MCH, and MCHC values in group 4 were greater, as were the WBC levels in groups 4 and 6, whereas other groups had lower MCV and WBC values. The weekly body weight gain was insignificantly different between treatment groups. Throughout the experiment, none of the animals perished. Male rats\' liver, kidney, and heart underwent histopathological changes after ingesting curcumin, sodium nitrite, and anthocyanin.
CONCLUSIONS: Based on the findings, rats were more detrimental when curcumin, sodium nitrite, and anthocyanin were ingested together than when they were consumed individually, as evidenced by histopathological abnormalities in the liver, kidneys, and heart.
METHODS: For this purpose, forty-eight male Wistar rats were randomly divided into 8 groups (n = 6 each), encompassing untreated controls and experimental groups treated with curcumin, anthocyanins, and sodium nitrite. Three rats from each group were sacrificed by cervical dislocation under di-ethyl ether anesthesia after 2 and 4 weeks of therapy, respectively. Blood samples were collected for serum chemistry. All of the animals\' livers, hearts, and kidneys were removed and sent for histopathological examination.
RESULTS: After two weeks of inquiry, certain groups displayed higher hematological values, while others had lower values compared to the control group. AST, CK, and LDH enzyme activity were higher in groups 2-8, but urea concentrations were higher in groups 6 and 8. After four weeks, the Hb, MCH, and MCHC values in group 4 were greater, as were the WBC levels in groups 4 and 6, whereas other groups had lower MCV and WBC values. The weekly body weight gain was insignificantly different between treatment groups. Throughout the experiment, none of the animals perished. Male rats\' liver, kidney, and heart underwent histopathological changes after ingesting curcumin, sodium nitrite, and anthocyanin.
CONCLUSIONS: Based on the findings, rats were more detrimental when curcumin, sodium nitrite, and anthocyanin were ingested together than when they were consumed individually, as evidenced by histopathological abnormalities in the liver, kidneys, and heart.
摘要：
目的：本研究的目的是检查姜黄素的重复剂量28天口服毒性，花青素,Wistar大鼠体内的亚硝酸钠.
方法：为此，48只雄性Wistar大鼠随机分为8组(每组6只),包括未经治疗的对照组和用姜黄素治疗的实验组，花青素,和亚硝酸钠.治疗2周和4周后，每组3只大鼠在乙醚麻醉下颈椎脱位处死，分别。收集血液样品用于血清化学。所有的动物肝脏,心,肾脏被切除并送去组织病理学检查。
结果：经过两周的调查，某些组表现出更高的血液学值，而其他人的值低于对照组。AST,CK,LDH酶活性在2-8组中较高，而尿素浓度在6和8组中较高。四周后,Hb,MCH,第4组的MCHC值更高，第4组和第6组的WBC水平也是如此,而其他组的MCV和WBC值较低.治疗组之间的每周体重增加没有显著差异。在整个实验过程中,没有动物死亡。雄性大鼠肝脏,肾,摄入姜黄素后心脏发生了组织病理学变化，亚硝酸钠,还有花青素.
结论：根据调查结果，姜黄素对大鼠更有害，亚硝酸钠,和花青素一起摄入比单独食用时更多，肝脏的组织病理学异常证明了这一点，肾脏，和心脏。
方法：为此，48只雄性Wistar大鼠随机分为8组(每组6只),包括未经治疗的对照组和用姜黄素治疗的实验组，花青素,和亚硝酸钠.治疗2周和4周后，每组3只大鼠在乙醚麻醉下颈椎脱位处死，分别。收集血液样品用于血清化学。所有的动物肝脏,心,肾脏被切除并送去组织病理学检查。
结果：经过两周的调查，某些组表现出更高的血液学值，而其他人的值低于对照组。AST,CK,LDH酶活性在2-8组中较高，而尿素浓度在6和8组中较高。四周后,Hb,MCH,第4组的MCHC值更高，第4组和第6组的WBC水平也是如此,而其他组的MCV和WBC值较低.治疗组之间的每周体重增加没有显著差异。在整个实验过程中,没有动物死亡。雄性大鼠肝脏,肾,摄入姜黄素后心脏发生了组织病理学变化，亚硝酸钠,还有花青素.
结论：根据调查结果，姜黄素对大鼠更有害，亚硝酸钠,和花青素一起摄入比单独食用时更多，肝脏的组织病理学异常证明了这一点，肾脏，和心脏。
