Abstract：
BACKGROUND: Soluble inflammatory factors in the cerebrospinal fluid (CSF) of patients with neurosyphilis have been investigated with low-throughput technology. This study aimed to illustrate the characteristics of soluble factor profiles in CSF of patients with neurosyphilis.
METHODS: We measured the concentrations of 45 cytokines, chemokines, and growth factors in CSF from 112 untreated syphilis cases, including latent syphilis (LS), asymptomatic neurosyphilis (ANS), meningeal neurosyphilis (MNS), meningovascular neurosyphilis (MVNS), paralytic dementia (PD), and ocular syphilis (OS).
RESULTS: Thirty-three differentially expressed soluble factors (DeSFs) were categorized into 3 clusters. DeSF scores of clusters 1 and 2 (DeSFS1 and DeSFS2) were positively correlated with elevated neopterin and neurofilament light subunit (NF-L) concentration, respectively. DeSF scores of cluster 3 were positively correlated with white blood cells, protein, NF-L, and neopterin. Patients with LS, ANS, and OS exhibited an overall lower abundance of DeSFs. Patients with PD exhibited significantly increased levels of clusters 1 and 3, and the highest total DeSF score, whereas patients with MNS and MVNS showed enhanced levels of cluster 2. Receiver operating characteristic analysis revealed that DeSFS1 effectively discriminated PD, and DeSFS2 discriminated MNS/MVNS with high accuracy.
CONCLUSIONS: Patients with neurosyphilis at different stages have distinctive patterns of soluble factors in CSF, which are correlated with immune status and neuronal damage.
METHODS: We measured the concentrations of 45 cytokines, chemokines, and growth factors in CSF from 112 untreated syphilis cases, including latent syphilis (LS), asymptomatic neurosyphilis (ANS), meningeal neurosyphilis (MNS), meningovascular neurosyphilis (MVNS), paralytic dementia (PD), and ocular syphilis (OS).
RESULTS: Thirty-three differentially expressed soluble factors (DeSFs) were categorized into 3 clusters. DeSF scores of clusters 1 and 2 (DeSFS1 and DeSFS2) were positively correlated with elevated neopterin and neurofilament light subunit (NF-L) concentration, respectively. DeSF scores of cluster 3 were positively correlated with white blood cells, protein, NF-L, and neopterin. Patients with LS, ANS, and OS exhibited an overall lower abundance of DeSFs. Patients with PD exhibited significantly increased levels of clusters 1 and 3, and the highest total DeSF score, whereas patients with MNS and MVNS showed enhanced levels of cluster 2. Receiver operating characteristic analysis revealed that DeSFS1 effectively discriminated PD, and DeSFS2 discriminated MNS/MVNS with high accuracy.
CONCLUSIONS: Patients with neurosyphilis at different stages have distinctive patterns of soluble factors in CSF, which are correlated with immune status and neuronal damage.
摘要：
背景：已经使用低通量技术研究了神经梅毒患者脑脊液（CSF）中的可溶性炎症因子。本研究旨在说明神经梅毒患者脑脊液中可溶性因子谱的特征。
方法：我们测量了45种细胞因子的浓度，趋化因子,112例未经治疗的梅毒病例的脑脊液中的生长因子，包括潜伏梅毒（LS），无症状神经梅毒（ANS），脑膜神经梅毒（MNS），脑膜血管神经梅毒（MVNS），麻痹性痴呆(PD),和眼部梅毒（OS）。
结果：将33个差异表达的可溶性因子（DeSF）分为3个簇。组1和组2的DeSF评分(DeSFS1和DeSFS2)与新蝶呤和神经丝光亚基(NF-L)浓度升高呈正相关,分别。第3组的DeSF评分与白细胞呈正相关,蛋白质，NF-L,还有新蝶呤.LS患者,ANS,和OS表现出整体较低的DeSF丰度。PD患者表现出显著增加的簇1和3的水平，和最高的总DeSF评分，而MNS和MVNS患者显示第2组水平增强。接收机工作特性分析表明，DeSFS1有效地区分了PD，DeSFS2对MNS/MVNS的判别精度较高。
结论：不同阶段的神经梅毒患者在CSF中具有不同的可溶性因子模式，这与免疫状态和神经元损伤有关。
方法：我们测量了45种细胞因子的浓度，趋化因子,112例未经治疗的梅毒病例的脑脊液中的生长因子，包括潜伏梅毒（LS），无症状神经梅毒（ANS），脑膜神经梅毒（MNS），脑膜血管神经梅毒（MVNS），麻痹性痴呆(PD),和眼部梅毒（OS）。
结果：将33个差异表达的可溶性因子（DeSF）分为3个簇。组1和组2的DeSF评分(DeSFS1和DeSFS2)与新蝶呤和神经丝光亚基(NF-L)浓度升高呈正相关,分别。第3组的DeSF评分与白细胞呈正相关,蛋白质，NF-L,还有新蝶呤.LS患者,ANS,和OS表现出整体较低的DeSF丰度。PD患者表现出显著增加的簇1和3的水平，和最高的总DeSF评分，而MNS和MVNS患者显示第2组水平增强。接收机工作特性分析表明，DeSFS1有效地区分了PD，DeSFS2对MNS/MVNS的判别精度较高。
结论：不同阶段的神经梅毒患者在CSF中具有不同的可溶性因子模式，这与免疫状态和神经元损伤有关。
