Abstract：
Moringa oleifera Lam. leaves contain various nutrients and bioactive compounds. The present study aimed to assess the anti-fatigue capacity of a flavonoids concentrate purified from M. oleifera Lam. leaves. The total flavonoids in the purified extract were analyzed by ultra-performance liquid chromatography electrospray ionization tandem mass spectrometry (UPLC-MS/MS). The mice were supplemented with purified M. oleifera Lam. leaf flavonoid-rich extract (MLFE) for 14 days. The weight-loaded forced swimming test was used for evaluating exercise endurance. The 90-min non-weight-bearing swimming test was carried out to assess biochemical biomarkers correlated to fatigue and energy metabolism. UPLC-MS/MS analysis identified 83 flavonoids from MLFE. MLFE significantly increased the swimming time by 60%. Serum lactate (9.9 ± 0.9 vs. 8.9 ± 0.7), blood urea nitrogen (BUN) (8.8 ± 0.8 vs. 7.2 ± 0.5), and nonesterified fatty acid (NEFA) (2.4 ± 0.2 vs. 1.7 ± 0.3) were significantly elevated; phosphoenolpyruvate carboxykinase (PEPCK), glucokinase (GCK), and nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA expression were significantly downregulated; and heme oxygenase 1 mRNA expression was significantly upregulated in muscle after swimming. MLFE supplement significantly decreased serum lactate (8.0 ± 1.0 vs. 9.9 ± 0.9), BUN (8.6 ± 0.4 vs. 8.9 ± 0.8), and NEFA (2.3 ± 0.4 vs. 2.4 ± 0.2) and increased the protein and mRNA expression of GCK, PEPCK, and Nrf2. The enhancement of glucose metabolism and antioxidant function by MLFE contributes partly to its anti-fatigue action.
摘要：
辣木。叶子含有各种营养物质和生物活性化合物。本研究旨在评估从油茶中纯化的类黄酮浓缩物的抗疲劳能力。叶子。采用超高效液相色谱-电喷雾串联质谱(UPLC-MS/MS)分析纯化提取物中的总黄酮。小鼠补充有纯化的油菌。叶类黄酮丰富的提取物（MLFE）14天。使用负重强迫游泳测试来评估运动耐力。进行了90分钟的非负重游泳测试，以评估与疲劳和能量代谢相关的生化生物标志物。UPLC-MS/MS分析从MLFE中鉴定出83种类黄酮。MLFE显著增加了60%的游泳时间。血清乳酸（9.9±0.9vs.8.9±0.7),血尿素氮（BUN）（8.8±0.8vs.7.2±0.5),和非酯化脂肪酸（NEFA）（2.4±0.2vs.1.7±0.3）显着升高；磷酸烯醇丙酮酸羧激酶（PEPCK），葡萄糖激酶（GCK），和核因子红系2相关因子2（Nrf2）mRNA表达显着下调；游泳后肌肉中血红素加氧酶1mRNA表达显着上调。MLFE补充显着降低血清乳酸（8.0±1.0vs.9.9±0.9),BUN（8.6±0.4vs.8.9±0.8),和NEFA（2.3±0.4vs.2.4±0.2）并增加GCK的蛋白和mRNA表达，PEPCK,Nrf2MLFE对葡萄糖代谢和抗氧化功能的增强部分有助于其抗疲劳作用。
