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Abstract：
The aim of this study was to evaluate the antiproliferative properties of low-level laser therapy (LLLT) on gingival fibroblasts obtained from calcium channel blocker-induced gingival overgrowth (GO). Gingival fibroblasts of patients with GO were compared to healthy gingival fibroblasts (H). Both cells were exposed to LLLT (685 nm wavelength, 25mW power, diode laser) and compared to those not treated with LLLT. Cell proliferation and viability were measured with MTT assay at baseline and after 24 and 72 h. TGF-β1, CTGF, and collagen Type 1 levels were evaluated with Enzyme-Linked Immunosorbent Assay (ELISA). LLLT significantly decreased the proliferation of GO fibroblasts (p < 0.05) while leading to a significantly higher proliferation in H fibroblasts compared to the untreated cells (p < 0.05). GO cells showed significantly higher CTGF, TGF-β, and collagen Type 1 expression than the H cells (p < 0.05). LLLT significantly reduced CTGF levels in GO cells compared to the control group (p < 0.05). In H cells, CTGF and TGF-β levels were also significantly decreased in response to LLLT compared to the control group (p < 0.05). While LLLT significantly reduced collagen expression in the H group (p < 0.05), it did not significantly impact the GO cells. LLLT significantly reduced the synthesis of the growth factors and collagen in both groups with an antiproliferative effect on the gingival fibroblasts from calcium channel blocker-induced GO, suggesting that it can offer a therapeutic approach in the clinical management of drug-induced GO, reversing the fibrotic changes.
摘要：
这项研究的目的是评估低水平激光治疗（LLLT）对从钙通道阻断剂诱导的牙龈过度生长（GO）获得的牙龈成纤维细胞的抗增殖特性。将GO患者的牙龈成纤维细胞与健康牙龈成纤维细胞(H)进行比较。两个细胞都暴露于LLLT（685nm波长，25mW功率，二极管激光器），并与未经LLLT治疗的激光器进行比较。在基线以及24和72h后，用MTT测定法测量细胞增殖和活力。TGF-β1，CTGF，用酶联免疫吸附测定(ELISA)评价1型胶原水平。与未处理的细胞相比,LLLT显著降低GO成纤维细胞的增殖(p<0.05),同时导致H成纤维细胞中显著更高的增殖(p<0.05)。GO细胞显示明显较高的CTGF，TGF-β,1型胶原表达高于H细胞(p<0.05)。与对照组相比,LLLT显著降低GO细胞中的CTGF水平(p<0.05)。在H细胞中，与对照组相比,响应LLLT的CTGF和TGF-β水平也显著降低(p<0.05)。而LLLT显著降低H组胶原表达(p<0.05),它没有显着影响GO细胞。LLLT显着降低了两组中生长因子和胶原蛋白的合成，对钙通道阻断剂诱导的GO的牙龈成纤维细胞具有抗增殖作用，这表明它可以在药物诱导GO的临床管理中提供一种治疗方法，逆转纤维化的变化。
