Abstract：
Curcumin serves as a photosensitizer (PS) in the context of microbial inactivation when subjected to light exposure, to produce reactive oxygen species, which exhibit efficacy in eradicating microorganisms. This remarkable property underscores the growing potential of antimicrobial photodynamic therapy (aPDT) in the ongoing fight against bacterial infections. Considering this, we investigate the efficacy of various in vitro curcumin formulations within a PDT protocol designed to target Staphylococcus aureus. Specifically, we conduct a comparative analysis involving synthetic curcumin (Cur-Syn) and curcumin derivatives modified with chlorine (Cl), selenium (Se), and iodine (I) (Cur-Cl, Cur-Se, Cur-I). To assess the impact of aPDT, we subject S. aureus to incubation with curcumin, followed by irradiation at 450 nm with energy doses of 3.75, 7.5, and 15 J/cm2. Our investigation encompasses an evaluation of PS uptake and photobleaching across the various curcumin variants. Notably, all three modifications (Cur-Cl, Cur-Se, Cur-I) induce a significant reduction in bacterial viability, approximately achieving a 3-log reduction. Interestingly, the uptake kinetics of Cur-Syn and Cur-Se exhibit similarities, reaching saturation after 20 min. Our findings suggest that modifications to curcumin have a discernible impact on the photodynamic properties of the PS molecule.
摘要：
姜黄素作为光敏剂（PS）在微生物灭活时，产生活性氧，表现出根除微生物的功效。这种显著的特性强调了抗微生物光动力疗法(aPDT)在对抗细菌感染的持续斗争中的增长潜力。考虑到这一点,我们研究了针对金黄色葡萄球菌的PDT方案中各种姜黄素体外制剂的疗效.具体来说,我们进行了比较分析，涉及合成姜黄素（Cur-Syn）和用氯（Cl）修饰的姜黄素衍生物，硒(Se),和碘(I)(Cur-Cl，Cur-Se,Cur-I).为了评估aPDT的影响，我们让金黄色葡萄球菌与姜黄素孵育，然后用3.75、7.5和15J/cm2的能量剂量在450nm照射。我们的调查包括对各种姜黄素变体的PS摄取和光漂白的评估。值得注意的是,所有三个修改(Cur-Cl，Cur-Se,Cur-I)诱导细菌活力显著降低，大约实现3个对数的减少。有趣的是,Cur-Syn和Cur-Se的吸收动力学表现出相似性，20分钟后达到饱和。我们的发现表明，姜黄素的修饰对PS分子的光动力学特性具有明显的影响。
