METHODS: Serum immunoglobin (Ig) G antibodies against CMV and EBV were measured in patients with NMOSD and healthy controls (HCs), including anti-CMV, anti-EBV nuclear antigen-1 (EBNA-1), anti-EBV virus capsid antigen (VCA), and anti-EBV early antigen (EA) IgGs. The immune status ratio (ISR) was used to evaluate the serum anti-CMV and anti-EBV IgG levels and ISR ≧1.10 was defined as seropositivity.
RESULTS: In total, 238 serum samples were collected from 94 patients with NMOSD and 144 HCs, and no significant difference of sex and age between NMOSD and HCs. Comparing to the HCs, patients with NMOSD exhibited significantly higher serum anti-CMV IgG level. In contrast, the serum anti-EBNA1 IgG level was significantly lower in patients with NMOSD than in HCs. The serum anti-VCA and anti-EA IgG levels did not differ between the two groups, but the anti-EA seropositivity was significantly higher in NMOSD group than that in HC group. We did not find associations between serum anti-CMV or anti-EBV IgG levels and NMOSD disease stage, immunotherapy, or disability score.
CONCLUSIONS: Our findings indicated that increased CMV infection and EBV recent infection, as well as reduced EBV latency infection were associated with the risk of NMOSD. Prospective cohort studies are needed to verify our findings and clarify the correlation between CMV and EBV infections and clinical characteristics of NMOSD.
方法:在NMOSD患者和健康对照(HCs)中测量血清抗CMV和EBV的免疫球蛋白(Ig)G抗体,包括抗CMV,抗EBV核抗原-1(EBNA-1),抗EBV病毒衣壳抗原(VCA),和抗EBV早期抗原(EA)IgG。免疫状态比(ISR)用于评估血清抗CMV和抗EBVIgG水平,ISR彡1.10被定义为血清阳性。
结果:总计,从94例NMOSD和144例HCs患者中收集238份血清样本,NMOSD和HCs在性别和年龄上无显著差异。与HC相比,NMOSD患者血清抗CMVIgG水平显著升高.相比之下,NMOSD患者的血清抗EBNA1IgG水平明显低于HCs。两组血清抗VCA和抗EAIgG水平无差异,但NMOSD组的抗EA血清阳性率明显高于HC组。我们没有发现血清抗CMV或抗EBVIgG水平与NMOSD疾病分期之间的关联,免疫疗法,或残疾评分。
结论:我们的研究结果表明,CMV感染和EBV近期感染增加,以及减少EBV潜伏期感染与NMOSD风险相关。需要前瞻性队列研究来验证我们的发现,并阐明CMV和EBV感染与NMOSD临床特征之间的相关性。