Abstract：
BACKGROUND: MiR-486-5p has been identified as a crucial regulator of the PI3K/AKT signalling pathway, which plays a significant role in skeletal muscle development. Its host gene, sANK1, is also essential for skeletal muscle development. However, the understanding of porcine miR-486-5p and sANK1 has been limited.
RESULTS: In this study, PCR analyses revealed a positive correlation between the expression of miR-486-5p and sANK1 in the longissimus dorsi muscle of the Bama mini-pig and Landrace-pig, as well as during myoblast differentiation. Furthermore, the expression of miR-486-5p/sANK1 was higher in the Bama mini-pig compared to the Landrace-pig. There was a total of 18 single nucleotide polymorphisms (SNP) present in the sANK1 promoter region. Among these SNPs, 14 of them resulted in alterations in transcription factor binding sites (TFBs). Additionally, the promoter fluorescence assay demonstrated that the activity of the sANK1 promoter derived from the Bama mini-pig was significantly higher compared to Landrace-pig. It is worth noting that ten regulatory SNPs have the potential to influence the activity of the sANK1 promoter. A nuclear mutation A-G located at position - 401 (relative to the transcription start site) in the Bama mini-pig was identified, which creates a putative TFB motif for MyoD.
CONCLUSIONS: The findings presented in this study offer fundamental molecular knowledge and expression patterns of miR-486-5p/sANK1, which can be valuable for gaining a deeper understanding of the gene\'s involvement in porcine skeletal muscle development, and meat quality.
RESULTS: In this study, PCR analyses revealed a positive correlation between the expression of miR-486-5p and sANK1 in the longissimus dorsi muscle of the Bama mini-pig and Landrace-pig, as well as during myoblast differentiation. Furthermore, the expression of miR-486-5p/sANK1 was higher in the Bama mini-pig compared to the Landrace-pig. There was a total of 18 single nucleotide polymorphisms (SNP) present in the sANK1 promoter region. Among these SNPs, 14 of them resulted in alterations in transcription factor binding sites (TFBs). Additionally, the promoter fluorescence assay demonstrated that the activity of the sANK1 promoter derived from the Bama mini-pig was significantly higher compared to Landrace-pig. It is worth noting that ten regulatory SNPs have the potential to influence the activity of the sANK1 promoter. A nuclear mutation A-G located at position - 401 (relative to the transcription start site) in the Bama mini-pig was identified, which creates a putative TFB motif for MyoD.
CONCLUSIONS: The findings presented in this study offer fundamental molecular knowledge and expression patterns of miR-486-5p/sANK1, which can be valuable for gaining a deeper understanding of the gene\'s involvement in porcine skeletal muscle development, and meat quality.
摘要：
背景：MiR-486-5p已被确定为PI3K/AKT信号通路的关键调节因子，在骨骼肌发育中起着重要作用。它的宿主基因，sANK1也是骨骼肌发育所必需的。然而,对猪miR-486-5p和sANK1的了解有限。
结果：在这项研究中，PCR分析显示miR-486-5p和sANK1在巴马小型猪和长白猪背最长肌中的表达呈正相关,以及在成肌细胞分化过程中。此外，miR-486-5p/sANK1在巴马小型猪中的表达高于长白猪。在sANK1启动子区域中存在总共18个单核苷酸多态性(SNP)。在这些SNP中，其中14个导致转录因子结合位点(TFB)的改变。此外,启动子荧光分析表明，与长白猪相比，来自巴马小型猪的sANK1启动子的活性明显更高。值得注意的是,10个调节性SNP具有影响sANK1启动子活性的潜力。鉴定了巴马小型猪中位于位置-401（相对于转录起始位点）的核突变A-G，它为MyoD创建了一个假定的TFB基序。
结论：本研究中的发现提供了miR-486-5p/sANK1的基本分子知识和表达模式，这对于更深入地了解该基因参与猪骨骼肌发育是有价值的。和肉的质量。
结果：在这项研究中，PCR分析显示miR-486-5p和sANK1在巴马小型猪和长白猪背最长肌中的表达呈正相关,以及在成肌细胞分化过程中。此外，miR-486-5p/sANK1在巴马小型猪中的表达高于长白猪。在sANK1启动子区域中存在总共18个单核苷酸多态性(SNP)。在这些SNP中，其中14个导致转录因子结合位点(TFB)的改变。此外,启动子荧光分析表明，与长白猪相比，来自巴马小型猪的sANK1启动子的活性明显更高。值得注意的是,10个调节性SNP具有影响sANK1启动子活性的潜力。鉴定了巴马小型猪中位于位置-401（相对于转录起始位点）的核突变A-G，它为MyoD创建了一个假定的TFB基序。
结论：本研究中的发现提供了miR-486-5p/sANK1的基本分子知识和表达模式，这对于更深入地了解该基因参与猪骨骼肌发育是有价值的。和肉的质量。
