Abstract：
Six novel bacterial strains, designated N016T, N017, N022T, N028, N056T, and N064, were isolated from soil sampled on the Qinghai-Tibet Plateau. Cells were aerobic, orange or yellow, globular or rod-shaped, non-motile, non-spore-forming, Gram-stain-positive, catalase-positive and oxidase-negative. All the isolates were salt-tolerant and could grow in the range of 4-42 °C. Results of phylogenomic analyses based on 16S rRNA gene sequences and core genomic genes showed that the three pairs of strains (N016T/N017, N022T/N028, and N056T/N064) were closely related to the members of the genus Planococcus, and clustered with Planococcus ruber, Planococcus glaciei, and Planococcus chinensis. The digital DNA-DNA hybridization and average nucleotide identity values of the six novel strains with other members of the genus Planococcus were within the ranges of 18.7-53 % and 70.58-93.49 %, respectively, all below the respective recommended thresholds of 70.0 % and 95-96 %. The genomic DNA G+C content of the six strains ranged from 43.5 to 46.0 mol%. The major fatty acids of the six strains were anteiso-C15 : 0, iso-C14 : 0, and C16 : 1 ω7c alcohol. The predominant polar lipids of strains N016T, N022T, and N056T were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. Menaquinones 7 and 8 were the respiratory quinones. The results of the above analyses indicated that the six strains represent three novel species of the genus Planococcus, for which the names Planococcus shenhongbingii sp. nov. (type strain N016T=GDMCC 1.4062T=JCM 36224T), Planococcus shixiaomingii sp. nov. (type strain N022T=GDMCC 1.4063T=JCM 36225T), and Planococcus liqunii sp. nov. (type strain N056T=GDMCC 1.4064T=JCM 36226T) are proposed.
摘要：
六种新型细菌菌株，指定N016T,N017,N022T,N028,N056T,和N064是从青藏高原采样的土壤中分离出来的。细胞是有氧的，橙色或黄色，球形或杆状，不活动，非孢子形成，革兰氏染色阳性,过氧化氢酶阳性和氧化酶阴性。所有分离物都是耐盐的，可以在4-42°C的范围内生长。基于16SrRNA基因序列和核心基因组基因的系统发育分析结果表明，三对菌株（N016T/N017，N022T/N028和N056T/N064）与植物球菌属成员密切相关，并聚集了鲁伯平球菌，冰川植物球菌，和中国活球菌。6个新菌株与其他植物球菌属的数字DNA-DNA杂交和平均核苷酸同一性值在18.7-53％和70.58-93.49％的范围内，分别,均低于各自的建议阈值70.0%和95-96%。6个菌株的基因组DNAGC含量范围为43.5至46.0mol％。六个菌株的主要脂肪酸是antiso-C15：0，iso-C14：0和C16：1ω7c醇。菌株N016T的主要极性脂质，N022T,N056T是二磷脂酰甘油，磷脂酰甘油，和磷脂酰乙醇胺。甲基萘醌7和8是呼吸醌。上述分析的结果表明，这6个菌株代表了平球菌属的三个新物种，为此，命名为申洪宾吉planococussp。11月。（类型应变N016T=GDMCC1.4062T=JCM36224T），石小明英植物球菌。11月。（类型应变N022T=GDMCC1.4063T=JCM36225T），和猪planococcussp。11月。(类型应变N056T=GDMCC1.4064T=JCM36226T)被提出。
