Abstract：
Haematitum is a commonly used mineral medicine. It is toxic, as recorded in the second volume of Chinese Materia Medica. Therefore, it should not be taken for a long time. In this study, the effects of Haematitum and calcined Haematitum on multiple organ injuries in mice were investigated, and the mechanism of the toxicity of the related organs was explored by metabolomics. The mice were randomly divided into the control group, Haematitum low-dose group(ZS-L group), Haematitum high-dose group(ZS-H group), and calcined Haematitum high-dose group(DZS-H group), with 12 mice in each group. Haematitum decoction was given by continuous intragastric administration for 10 days. Then the life situation was observed, and samples were taken to detect various indicators. The results showed that the ZS-H group showed obvious toxicity, with different degrees of toxicity damage in the intestinal tract,liver, spleen, and lung. ZS-L group had no toxic reaction. The toxicity of the DZS-H group was significantly reduced, and only the lung was damaged. Metabolomics technology was used to detect the lung tissue of mice in the control group and the ZS-H group, and a total of 15 kinds of significant difference metabolites were detected, mainly involved in choline metabolism in cancer, sphingolipid metabolism, and glycerophospholipid metabolism. Immunohistochemical results showed that the INSIG1 protein expression level in the lung tissue of mice in the ZS-H group was significantly higher than that in the control group. In summary, large doses and long-time use of Haematitum decoction will cause a variety of organ damage, and the same dose of calcined Haematitum is less toxic than Haematitum. In addition, a low dose of Haematitum has no obvious toxic effect. The dysfunction of lipid metabolic pathways such as sphingolipid and glycerophospholipid metabolism may be an important factor in Haematitum-induced pulmonary toxicity. This study provides a reference for further research on the mechanism of Haematitum pulmonary toxicity.
摘要：
血药是一种常用的矿物药物。它是有毒的，正如《中药》第二卷所记载的那样。因此,它不应该服用很长时间。在这项研究中,研究了血热和煅烧血热对小鼠多器官损伤的影响，并通过代谢组学探索相关器官的毒性机制。将小鼠随机分为对照组,血透低剂量组（ZS-L组），血液高剂量组(ZS-H组),煅烧血痰高剂量组（DZS-H组），每组12只小鼠。连续灌胃给药痰汤10天。然后观察生活状况，并取样检测各种指标。结果表明,ZS-H组具有明显的毒性,在肠道中具有不同程度的毒性损伤，肝脏,脾,脾还有肺.ZS-L组无毒性反应。DZS-H组的毒性显著降低,只有肺受损.采用代谢组学技术检测对照组和ZS-H组小鼠肺组织,共检测到15种显著差异的代谢物,主要参与癌症中的胆碱代谢，鞘脂代谢，和甘油磷脂代谢。免疫组织化学结果显示,ZS-H组小鼠肺组织中INSIG1蛋白表达水平明显高于对照组。总之,大剂量和长时间使用血药汤会引起多种器官损伤,和相同剂量的煅烧血药水比血药水毒性小。此外,低剂量的Haematum没有明显的毒性作用。鞘脂和甘油磷脂代谢等脂质代谢途径的功能障碍可能是血痰引起的肺毒性的重要因素。本研究为进一步研究血痰肺毒性机制提供了参考。
