Abstract:
This study aims to reveal the differences in the species and relative content of metabolites in the leaf and root tuber of Fallopia multiflora and improve the comprehensive utilization rate of F. multiflora resources. The metabolites in the root tubers and leaves of F. multiflora were detected by widely targeted metabolomics based on ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). The principal component analysis, hierarchical cluster analysis, and orthogonal partial least squares-discriminant analysis were carried out to screen the differential metabolites between the leaf and root tuber of F. multiflora. The result showed that a total of 1 942 metabolites in 15 categories were detected in the leaf and root tuber of F. multiflora, including 1 861 metabolites in the root tuber, 1 901 metabolites in the leaf, and 1 820 metabolites in both. The metabolites were mainly phenolic acids, flavonoids, amino acids and derivatives, and alkaloids. A total of 1 200 differential metabolites were screened out, accounting for 65.9% of the total metabolites. Among these differential metabolites, 813 and 387 showed higher content in the leaf and root tuber, respectively. Flavonoids were the metabolites with the largest number and the most significant differences between the leaf and root tuber, and stilbenes and anthraquinones as the main active compounds mainly existed in the root tuber. The KEGG enrichment results suggested that the differential metabolites were mainly enriched in flavonoid and flavonol biosynthesis pathways and linoleic acid metabolism pathway. This study discovered abundant metabolites in F. multiflora. The metabolites were similar but had great differences in the content between the leaf and root tuber. The research results provide theoretical guidance for the development and utilization of F. multiflora resources.
摘要:
本研究旨在揭示多花Fallopia叶片和块根代谢产物的种类和相对含量的差异,提高Fallopia资源的综合利用率。基于超高效液相色谱-串联质谱(UPLC-MS/MS)技术,采用广泛靶向代谢组学技术对多花草块茎和叶片中的代谢产物进行检测。主成分分析,层次聚类分析,采用正交偏最小二乘-判别分析法筛选多花叶根块茎的差异代谢产物。结果表明,在多花F的叶片和块根中共检测到15个类别的1942种代谢物,块根中包括1861种代谢物,叶中的1901种代谢物,和1820种代谢物。代谢产物主要为酚酸,黄酮类化合物,氨基酸及其衍生物,和生物碱。共筛选出1200种差异代谢物,占代谢物总量的65.9%。在这些不同的代谢物中,813和387在块茎叶和根中显示出更高的含量,分别。黄酮类化合物是块茎叶和根之间数量最多、差异最显著的代谢产物,二苯乙烯和蒽醌作为主要活性化合物主要存在于块茎中。KEGG富集结果表明,差异代谢产物主要富集在黄酮类和黄酮醇生物合成途径和亚油酸代谢途径。这项研究在多花菌中发现了丰富的代谢物。代谢产物相似,但在叶片和块根之间的含量差异很大。研究结果为多花叶草资源的开发利用提供了理论指导。