关键词: Endonuclease Expression In vitro Polymerase Purification RVF Replication

Mesh : Rift Valley fever virus / genetics Saccharomyces cerevisiae / genetics Viral Proteins / metabolism genetics Enzyme Assays / methods Humans Gene Expression Virus Replication / genetics

来  源:   DOI:10.1007/978-1-0716-3926-9_20

Abstract:
Rift Valley fever (RVF) virus is widespread worldwide and poses a severe threat to human life and property. RVF viral polymerase plays a vital role in the replication and transcription of the virus. Here, we describe how to express and purify this polymerase and perform tests for its in vitro activity assays.
摘要:
裂谷热(RVF)病毒在世界范围内普遍存在,对人类生命和财产构成严重威胁。RVF病毒聚合酶在病毒的复制和转录中起着至关重要的作用。这里,我们描述了如何表达和纯化这种聚合酶,并对其体外活性测定进行测试。
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