{Reference Type}: Journal Article {Title}: Yeast Expression and Activity Assay of RVFV Polymerase. {Author}: Wang X;Tang L;Chen E;Chen Z; {Journal}: Methods Mol Biol {Volume}: 2824 {Issue}: 0 {Year}: 2024 暂无{DOI}: 10.1007/978-1-0716-3926-9_20 {Abstract}: Rift Valley fever (RVF) virus is widespread worldwide and poses a severe threat to human life and property. RVF viral polymerase plays a vital role in the replication and transcription of the virus. Here, we describe how to express and purify this polymerase and perform tests for its in vitro activity assays.