Abstract：
Vector competence assays allow to measure, in the laboratory, the ability of a mosquito to get infected and then retransmit an arbovirus while mimicking natural vector infection route. Aedes aegypti is a major vector of arboviruses worldwide and thus a reference species used in vector competence assays. Rift Valley fever virus (RVFV) is a major public health threat, mostly in Africa, that infects humans and animals through the bite of mosquito vectors. Here, we describe vector competence assay of Aedes aegypti mosquitoes for RVFV, from mosquito exposure to the virus through an infectious artificial blood meal to the measurement of virus prevalence in the mosquito\'s body, head, and saliva.
摘要：
矢量能力测定允许测量，在实验室里,蚊子在模仿自然媒介感染途径的同时被感染然后再传播虫媒病毒的能力。埃及伊蚊是世界范围内虫媒病毒的主要载体，因此是载体能力测定中使用的参考物种。裂谷热病毒（RVFV）是一种主要的公共卫生威胁，主要在非洲，通过蚊虫叮咬感染人类和动物。这里,我们描述了埃及伊蚊对RVFV的载体能力测定，从蚊子通过感染性人工血餐接触病毒到测量蚊子体内的病毒流行率，头部,还有唾液.
