PDF(Pubmed)
Abstract：
UNASSIGNED: Interest in the safety of herbal medicine is growing rapidly regarding knowledge and challenges in natural products. Hence, this study aimed to reveal the toxicological profile of Ardisia elliptica, a traditional medicinal plant used in the treatment of various illnesses.
UNASSIGNED: Acute toxicity study was performed on female and male Sprague Dawley rats with a single oral administration of 2000 mg/kg BW of 70% ethanolic A. elliptica leaf extract, using a combination of conventional investigations and 1H-NMR-based metabolomics approaches.
UNASSIGNED: Physical, hematological, biochemical, and histopathological assessments demonstrated the usual rat profile, with no mortality and delayed toxicity 14 days after administration. 1H NMR serum metabolomics depicted similar metabolites between normal and treated groups. Nevertheless, 1H NMR of urinary metabolomics revealed perturbation in carbohydrate, amino acid, and energy metabolism within 24h after extract administration, while no accumulation of toxic biomarkers in the collected biological fluids on Day 14. A minor gender-based difference revealed the influence of sex hormones and different energy expenditure on response to extract treatment.
UNASSIGNED: This study suggested that 2000 mg/kg BW of 70% ethanolic A. elliptica leaf extract is considered as safe for consumption and offered a comprehensive overview of the response of physiological and metabolic aspects applicable to food and herbal product development.
UNASSIGNED: Acute toxicity study was performed on female and male Sprague Dawley rats with a single oral administration of 2000 mg/kg BW of 70% ethanolic A. elliptica leaf extract, using a combination of conventional investigations and 1H-NMR-based metabolomics approaches.
UNASSIGNED: Physical, hematological, biochemical, and histopathological assessments demonstrated the usual rat profile, with no mortality and delayed toxicity 14 days after administration. 1H NMR serum metabolomics depicted similar metabolites between normal and treated groups. Nevertheless, 1H NMR of urinary metabolomics revealed perturbation in carbohydrate, amino acid, and energy metabolism within 24h after extract administration, while no accumulation of toxic biomarkers in the collected biological fluids on Day 14. A minor gender-based difference revealed the influence of sex hormones and different energy expenditure on response to extract treatment.
UNASSIGNED: This study suggested that 2000 mg/kg BW of 70% ethanolic A. elliptica leaf extract is considered as safe for consumption and offered a comprehensive overview of the response of physiological and metabolic aspects applicable to food and herbal product development.
摘要：
■关于天然产品的知识和挑战，人们对草药安全性的兴趣正在迅速增长。因此，本研究旨在揭示椭圆型Ardisia的毒理学特征，一种用于治疗各种疾病的传统药用植物。
■对雌性和雄性SpragueDawley大鼠进行急性毒性研究，单次口服2000mg/kgBW的70％乙醇A。椭圆叶提取物，使用常规研究和基于1H-NMR的代谢组学方法的组合。
■物理，血液学,生物化学，和组织病理学评估显示了通常的大鼠特征，给药后14天没有死亡和延迟毒性。1HNMR血清代谢组学描绘了正常组和治疗组之间相似的代谢物。然而，尿代谢组学的1HNMR揭示了碳水化合物的扰动，氨基酸,提取物给药后24h内的能量代谢，而在第14天收集的生物体液中没有毒性生物标志物的积累。基于性别的微小差异揭示了性激素和不同能量消耗对提取物治疗反应的影响。
■这项研究表明，2000mg/kgBW的70％乙醇A.elliptica叶提取物被认为是安全的，并提供了适用于食品和草药产品开发的生理和代谢方面的反应的全面概述。
■对雌性和雄性SpragueDawley大鼠进行急性毒性研究，单次口服2000mg/kgBW的70％乙醇A。椭圆叶提取物，使用常规研究和基于1H-NMR的代谢组学方法的组合。
■物理，血液学,生物化学，和组织病理学评估显示了通常的大鼠特征，给药后14天没有死亡和延迟毒性。1HNMR血清代谢组学描绘了正常组和治疗组之间相似的代谢物。然而，尿代谢组学的1HNMR揭示了碳水化合物的扰动，氨基酸,提取物给药后24h内的能量代谢，而在第14天收集的生物体液中没有毒性生物标志物的积累。基于性别的微小差异揭示了性激素和不同能量消耗对提取物治疗反应的影响。
■这项研究表明，2000mg/kgBW的70％乙醇A.elliptica叶提取物被认为是安全的，并提供了适用于食品和草药产品开发的生理和代谢方面的反应的全面概述。
