Abstract：
Implant-associated infections and excessive immune responses are two major postsurgical issues for successful implantation. However, conventional strategies including antibiotic treatment and inflammatory regulation are always compromised due to the comodification of various biochemical agents and instances of functional interference. It is imperative to provide implant surfaces with satisfactory antibacterial and anti-inflammatory properties. Here, a dual-effect nanostructured polyetheretherketone (PEEK) surface (NP@PDA/Zn) with bionic mechano-bactericidal nanopillars and immobilized immunomodulatory Zn2+ is designed. The constructed hybrid nanopillars display remarkable antibacterial performance against Gram-negative and Gram-positive strains through the synergy of physical and chemical bactericidal effects imposed by nanopillars and Zn2+. Meanwhile, the immunoregulatory property is evaluated through the investigation of macrophage polarization both in vitro and in vivo, and the results reveal that NP@PDA/Zn could downregulate the expression of M1-related cytokines and decrease the M1 macrophage recruitment to lower the inflammatory response. Notably, the surface exhibited exceptional biocompatibility with discerning biocidal activity between bacterial and mammalian cells and antioxidant performance that effectively scavenges ROS, minimizing potential cytotoxicity. Taken together, NP@PDA/Zn presents a convenient and promising strategy of combining synergistic bactericidal activity and inflammatory regulation without any mutual interference, which can support the development of multifunctional implant-associated materials.
摘要：
植入物相关的感染和过度的免疫反应是成功植入的两个主要的术后问题。然而,包括抗生素治疗和炎症调节在内的常规策略总是由于各种生化试剂的共同修饰和功能干扰而受到损害.必须提供具有令人满意的抗菌和抗炎特性的植入物表面。这里,设计了具有仿生机械杀菌纳米柱和固定化免疫调节Zn2的双效应纳米结构聚醚醚酮（PEEK）表面（NP@PDA/Zn）。通过纳米柱和Zn2施加的物理和化学杀菌作用的协同作用，构建的混合纳米柱对革兰氏阴性和革兰氏阳性菌株具有显着的抗菌性能。同时,免疫调节特性是通过研究巨噬细胞极化在体外和体内，结果表明，NP@PDA/Zn可以下调M1相关细胞因子的表达，减少M1巨噬细胞的募集，从而降低炎症反应。值得注意的是,表面表现出特殊的生物相容性与辨别细菌和哺乳动物细胞之间的杀生物活性和抗氧化性能，有效清除ROS，最小化潜在的细胞毒性。一起来看,NP@PDA/Zn提出了一种方便且有前途的策略，可以将协同杀菌活性和炎症调节相结合，而无需任何相互干扰。这可以支持多功能植入物相关材料的开发。
