Abstract：
Right up to now, there has not been an effective or safe therapy for trichinellosis. Thus, this study aimed to determine the efficacy of prophylactic and therapeutic regimens of progesterone and mifepristone on the intestinal and muscular phases of experimental Trichinella spiralis infection compared to albendazole. Seven distinct groups of mice were divided as follows: negative, positive, and drug control groups, as well as prophylactic and treatment groups using mifepristone and progesterone. Mice were sacrificed on the 7th and 37th days after infection. Treatment efficacy was evaluated using parasitological techniques, histopathological examination, immunohistochemical staining, and ultrastructural morphological analysis of adult worms by scanning electron microscopy. The mice groups received progesterone (300 ng/ml) and mifepristone (100 ng/ml). They demonstrated a significant improvement in intestinal and muscular inflammation and a statistically significant decline in the adult worm burden and encysted larvae (P < 0.001). Moreover, immunohistochemical staining of vascular endothelial growth factor and mucosal mast cell analyses were coincided with the obtained parasitological results. There was notable destruction and degeneration of the adult worm tegument by using both drugs. The current study pointed out that progesterone and mifepristone may provide new insights regarding the development of vaccines and drug protocols to treat trichinellosis through their combined action in reducing the inflammation, affecting the intestinal immune cell, and decreasing the adult worm burden, and larval capsule development.
摘要：
到现在为止,对于旋毛虫病，目前尚无有效或安全的治疗方法。因此，本研究旨在确定与阿苯达唑相比，孕酮和米非司酮对实验性旋毛虫感染的肠道和肌肉期的预防和治疗方案的疗效。七个不同组的小鼠分为如下：阴性，积极的,和药物对照组，以及使用米非司酮和孕酮的预防和治疗组。在感染后第7天和第37天处死小鼠。使用寄生虫学技术评估治疗效果，组织病理学检查,免疫组织化学染色，并通过扫描电子显微镜对成虫进行超微结构形态分析。小鼠组接受孕酮(300ng/ml)和米非司酮(100ng/ml)。他们证明了肠道和肌肉炎症的显着改善，并且成年蠕虫负担和包膜幼虫的统计学显着下降（P<0.001）。此外，血管内皮生长因子的免疫组织化学染色和粘膜肥大细胞分析与获得的寄生虫学结果一致。使用两种药物对成年蠕虫外皮都有明显的破坏和退化。目前的研究指出，孕酮和米非司酮可能会提供新的见解，关于疫苗和药物方案的发展，以治疗旋毛虫病，通过它们在减少炎症的联合作用，影响肠道免疫细胞，减轻成虫的负担，和幼虫囊发育。
