Abstract：
BACKGROUND: Schumanniophyton magnificum is a medicinal plant used to manage many ailments including malaria, skin diseases, parasitic infections, male sexual dysfunctions, female infertility and typhoid fever. However, no scientific investigation has been made for its folkloric use by the \"Baka\" Pygmies of Cameroon as an aphrodisiac.
OBJECTIVE: To investigate the aphrodisiac and androgenic activities of the aqueous extract of the roots of Schumanniophyton magnificum in male rats and analyze the phytoconstituents by UHPLC/MS.
METHODS: Twenty-five male rats of 16-weeks old were divided into 5 groups and orally treated for 30 days with distilled water (10 ml/kg), or sildenafil citrate (5 mg/kg), or the aqueous extract of Schumanniophyton magnificum (43 mg/kg, 86 mg/kg and 172 mg/kg). The sexual behaviour parameters were monitored on day 1 and 30 by pairing male rats to receptive females. At the end of the experiment, rats were killed and the blood and reproductive organs were collected for histological sectioning, sperm analysis and biochemical analysis. The presence of phytoconstituents and their structures were revealed by UHPLC/MS.
RESULTS: The plant extract significantly increased the mount, ejaculation and intromission frequencies in comparison to those in the normal control group; and significantly doubled the serum testosterone levels (2.15 ± 0.70 ng/ml) compared to the normal control group. UHPLC/MS of the aqueous extract of Schumanniophyton magnificum identified 7 major compounds such as Schumanniofioside A, Noreugenin and Rohitukine, with antioxidant and antibacterial activities. The plant extracts significantly increased the penile nitric oxide levels (P <0.05). These results were similar to those obtained after administration of sildenafil citrate.
CONCLUSIONS: The aqueous extract of Schumanniophyton magnificum could be an alternative for erectile dysfunction management.
OBJECTIVE: To investigate the aphrodisiac and androgenic activities of the aqueous extract of the roots of Schumanniophyton magnificum in male rats and analyze the phytoconstituents by UHPLC/MS.
METHODS: Twenty-five male rats of 16-weeks old were divided into 5 groups and orally treated for 30 days with distilled water (10 ml/kg), or sildenafil citrate (5 mg/kg), or the aqueous extract of Schumanniophyton magnificum (43 mg/kg, 86 mg/kg and 172 mg/kg). The sexual behaviour parameters were monitored on day 1 and 30 by pairing male rats to receptive females. At the end of the experiment, rats were killed and the blood and reproductive organs were collected for histological sectioning, sperm analysis and biochemical analysis. The presence of phytoconstituents and their structures were revealed by UHPLC/MS.
RESULTS: The plant extract significantly increased the mount, ejaculation and intromission frequencies in comparison to those in the normal control group; and significantly doubled the serum testosterone levels (2.15 ± 0.70 ng/ml) compared to the normal control group. UHPLC/MS of the aqueous extract of Schumanniophyton magnificum identified 7 major compounds such as Schumanniofioside A, Noreugenin and Rohitukine, with antioxidant and antibacterial activities. The plant extracts significantly increased the penile nitric oxide levels (P <0.05). These results were similar to those obtained after administration of sildenafil citrate.
CONCLUSIONS: The aqueous extract of Schumanniophyton magnificum could be an alternative for erectile dysfunction management.
摘要：
背景：Schumanniophytonmagnicum是一种药用植物，用于治疗包括疟疾在内的许多疾病，皮肤病,寄生虫感染,男性性功能障碍,女性不孕和伤寒。然而,喀麦隆的“巴卡”俾格米人没有对其民俗用途进行科学调查。
目的：研究雄鼠熊果根水提物的壮阳和雄性激素活性，并通过UHPLC/MS分析植物成分。
方法：将25只16周龄雄性大鼠分为5组，用蒸馏水（10ml/kg）口服30天，或枸橼酸西地那非（5mg/kg），或舒曼尼坦的水提取物(43毫克/千克，86mg/kg和172mg/kg）。通过将雄性大鼠与接受性雌性配对，在第1天和第30天监测性行为参数。实验结束时,处死大鼠，收集血液和生殖器官进行组织学切片，精子分析和生化分析。UHPLC/MS揭示了植物成分及其结构的存在。
结果：植物提取物显著增加了坐骑，与正常对照组相比，射精和射入频率；与正常对照组相比，血清睾酮水平显着提高了一倍（2.15±0.70ng/ml）。舒马尼磷的水提物的UHPLC/MS鉴定了7个主要化合物，例如舒马尼磷A，Noreugenin和Rohitukine,具有抗氧化和抗菌活性。植物提取物显着提高了阴茎一氧化氮水平（P=0.05）。这些结果与施用柠檬酸西地那非后获得的结果相似。
结论：舒马尼霉素的水提物可能是治疗勃起功能障碍的一种替代方法。
目的：研究雄鼠熊果根水提物的壮阳和雄性激素活性，并通过UHPLC/MS分析植物成分。
方法：将25只16周龄雄性大鼠分为5组，用蒸馏水（10ml/kg）口服30天，或枸橼酸西地那非（5mg/kg），或舒曼尼坦的水提取物(43毫克/千克，86mg/kg和172mg/kg）。通过将雄性大鼠与接受性雌性配对，在第1天和第30天监测性行为参数。实验结束时,处死大鼠，收集血液和生殖器官进行组织学切片，精子分析和生化分析。UHPLC/MS揭示了植物成分及其结构的存在。
结果：植物提取物显著增加了坐骑，与正常对照组相比，射精和射入频率；与正常对照组相比，血清睾酮水平显着提高了一倍（2.15±0.70ng/ml）。舒马尼磷的水提物的UHPLC/MS鉴定了7个主要化合物，例如舒马尼磷A，Noreugenin和Rohitukine,具有抗氧化和抗菌活性。植物提取物显着提高了阴茎一氧化氮水平（P=0.05）。这些结果与施用柠檬酸西地那非后获得的结果相似。
结论：舒马尼霉素的水提物可能是治疗勃起功能障碍的一种替代方法。
