Abstract：
Hemoglobin (Hb)-based oxygen carriers are investigated as a potential alternative or supplement to regular blood transfusions, particularly in critical and life-threatening scenarios. These include situations like severe trauma in remote areas, battlefield conditions, instances where blood transfusion is not feasible due to compatibility concerns, or when patients decline transfusions based on religious beliefs. This study introduces a novel method utilizing poly(ethylene glycol) (PEG) to entrap Hb within ZIF-8 nanoparticles (i.e., Hb@ZIF-8 NPs). Through meticulous screening, we achieved Hb@ZIF-8 NPs with a record-high Hb concentration of 34 mg mL-1. These NPs, sized at 168 nm, displayed exceptional properties: a remarkable 95 % oxyhemoglobin content, excellent encapsulation efficiency of 85 %, and resistance to Hb oxidation into methemoglobin (metHb). The addition of PEG emerged as a crucial factor amplifying Hb entrapment within ZIF-8, especially at higher Hb concentrations, reaching an unprecedented 34 mg mL-1. Importantly, PEG exhibited a protective effect, preventing metHb conversion in Hb@ZIF-8 NPs at elevated Hb concentrations.
摘要：
血红蛋白（Hb）为基础的氧载体被研究作为一个潜在的替代或补充定期输血，特别是在危急和危及生命的情况下。这些包括偏远地区的严重创伤，战场条件,由于兼容性问题，输血不可行的情况，或者当患者基于宗教信仰拒绝输血时。本研究介绍了一种利用聚(乙二醇)(PEG)将Hb包埋在ZIF-8纳米颗粒中的新方法(即，Hb@ZIF-8NPs)。通过细致的筛选,我们获得了Hb@ZIF-8NP，Hb浓度达到创纪录的34mgmL-1。这些NP,尺寸为168nm，表现出卓越的特性：显著的95%氧合血红蛋白含量，优异的封装效率为85%，以及对Hb氧化为高铁血红蛋白(metHb)的抗性。PEG的添加成为放大ZIF-8内Hb截留的关键因素，尤其是在较高的Hb浓度下，达到前所未有的34mgmL-1。重要的是,PEG表现出保护作用，在升高的Hb浓度下，防止Hb@ZIF-8NP中的metHb转化。
