PDF(Pubmed)
Abstract：
According to the Domestic Animal Diversity Information System (DAD-IS) of the FAO, Italy has one of the largest numbers of local small ruminant breeds among European countries. In Southern Italy, namely the Campania Region, Bagnolese and Laticauda sheep breeds and Cilentana goat breeds are considered endangered according to the DAD-IS. Conservation of endangered animal breeds is a goal of the European Union (EU). However, the role of infectious diseases as risk factors for endangered breeds has rarely been considered. Small ruminant lentiviruses (SRLV) infect sheep and goats, causing slow-progressive, persistent, and debilitating diseases that can lead to animal death and productivity loss. In this study, we investigated the presence of SRLV in Bagnolese, Laticauda, and Cilentana breeds using a commercial ELISA in parallel with an in-house ELISA. The results of the two tests were in good agreement (Cohen Kappa 0.84, 95 % CI = 0.76-0.93). Discrepancies between the two tests were resolved using western blotting. In total, 430 samples were tested (248 Bagnolese, 125 Laticauda, and 57 Cilentana). The apparent prevalence rates were 12.5 %, 6.4 %, and 1.7 % in Bagnolese, Laticauda, and Cilentana, respectively. In the molecular analysis of 11 proviral partial sequences, subtypes B2 and A24 were identified in two Bagnolese herds. Owing to the beneficial role of sheep and goat breeding in marginal areas, it is important to screen the entire population and implement control/eradication of SRLV infections in conjunction with each conservation program.
摘要：
根据粮农组织的国内动物多样性信息系统（DAD-IS），意大利是欧洲国家中当地小反刍动物品种数量最多的国家之一。在意大利南部,即坎帕尼亚地区，根据DAD-IS，Bagnolese和Laticauda绵羊品种和Cilentana山羊品种被认为濒临灭绝。保护濒危动物品种是欧盟（EU）的目标。然而,传染病作为濒危品种的危险因素的作用很少被考虑。小反刍动物慢病毒（SRLV）感染绵羊和山羊，导致缓慢的进展，持久性,以及导致动物死亡和生产力损失的衰弱疾病。在这项研究中,我们调查了Bagnolese中SRLV的存在，Laticauda,和Cilentana品种使用商业ELISA与内部ELISA平行。两项测试的结果吻合良好（CohenKappa0.84，95％CI=0.76-0.93）。使用蛋白质印迹解决两个测试之间的差异。总的来说,对430个样本进行了测试(248个Bagnolese，125Laticauda,和57Cilentana）。表观患病率为12.5%,6.4%,和1.7%的Bagnolese，Laticauda,还有Cilentana,分别。在11个前病毒部分序列的分子分析中，在两个Bagnolese牛群中鉴定出B2和A24亚型。由于边缘地区绵羊和山羊育种的有益作用，重要的是要筛查整个人群，并结合每个保护计划实施SRLV感染的控制/根除。
