PDF(Pubmed)
Abstract：
Aspergillus flavus is a clinically and agriculturally important saprotrophic fungus responsible for severe human infections and extensive crop losses. We analyzed genomic data from 250 (95 clinical and 155 environmental) A. flavus isolates from 9 countries, including 70 newly sequenced clinical isolates, to examine population and pan-genome structure and their relationship to pathogenicity. We identified five A. flavus populations, including a new population, D, corresponding to distinct clades in the genome-wide phylogeny. Strikingly, > 75% of clinical isolates were from population D. Accessory genes, including genes within biosynthetic gene clusters, were significantly more common in some populations but rare in others. Population D was enriched for genes associated with zinc ion binding, lipid metabolism, and certain types of hydrolase activity. In contrast to the major human pathogen Aspergillus fumigatus, A. flavus pathogenicity in humans is strongly associated with population structure, making it a great system for investigating how population-specific genes contribute to pathogenicity.
摘要：
黄曲霉是一种临床和农业上重要的腐生真菌，可导致严重的人类感染和广泛的作物损失。我们分析了来自9个国家的250个(95个临床和155个环境)黄曲霉分离株的基因组数据，包括70个新测序的临床分离株,研究群体和泛基因组结构及其与致病性的关系。我们确定了五个黄曲霉种群，包括新的人口，D,对应于全基因组系统发育中的不同进化枝。引人注目的是，>75%的临床分离株来自D群体。包括生物合成基因簇中的基因，在某些人群中明显更常见，但在其他人群中很少见。群体D富集了与锌离子结合相关的基因，脂质代谢，和某些类型的水解酶活性。与人类主要病原体烟曲霉相反，黄曲霉在人类中的致病性与种群结构密切相关，使其成为研究群体特异性基因如何导致致病性的一个很好的系统。
