PDF(Pubmed)
Abstract：
To understand the genomic characteristics of Arctic plants, we generated 28-44 Gb of short-read sequencing data from 13 Arctic plants collected from the High Arctic Svalbard. We successfully estimated the genome sizes of eight species by using the k-mer-based method (180-894 Mb). Among these plants, the mountain sorrel (Oxyria digyna) and Greenland scurvy grass (Cochlearia groenlandica) had relatively small genome sizes and chromosome numbers. We obtained 45 × and 121 × high-fidelity long-read sequencing data. We assembled their reads into high-quality draft genomes (genome size: 561 and 250 Mb; contig N50 length: 36.9 and 14.8 Mb, respectively), and correspondingly annotated 43,105 and 29,675 genes using ~46 and ~85 million RNA sequencing reads. We identified 765,012 and 88,959 single-nucleotide variants, and 18,082 and 7,698 structural variants (variant size ≥ 50 bp). This study provided high-quality genome assemblies of O. digyna and C. groenlandica, which are valuable resources for the population and molecular genetic studies of these plants.
摘要：
了解北极植物的基因组特征，我们从高北极斯瓦尔巴特群岛收集的13种北极植物产生了28-44Gb的短读数测序数据。我们通过使用基于k-mer的方法（180-894Mb）成功地估计了8个物种的基因组大小。在这些植物中,山sorrel（Oxyriadigyna）和格陵兰镰刀草（Cochleariagroenlandica）的基因组大小和染色体数量相对较小。我们获得了45倍和121倍高保真长读测序数据。我们将他们的读段组装成高质量的草图基因组（基因组大小：561和250Mb；重叠群N50长度：36.9和14.8Mb，分别)，并使用〜46和〜8500万个RNA测序读数相应地注释了43,105和29,675个基因。我们鉴定了765,012和88,959个单核苷酸变体，以及18,082和7,698个结构变体（变体大小≥50bp）。这项研究提供了Odigyna和C.groenlandica的高质量基因组组装，它们是这些植物种群和分子遗传研究的宝贵资源。
