Abstract：
Blood samples from fifteen captive Indian wolves (Canis lupus pallipes) maintained at Arignar Anna Zoological Park, Vandalur, Chennai were screened for the presence of Babesia spp., Ehrlichia canis and Trypnosoma evansi DNA by PCR. Out of 15 wolf samples, 3 samples were found positive for Babesia spp. The amplified 18S rRNA gene fragments from 3 wolves were sequenced and confirmed as Babesia gibsoni. A maximum likelihood tree was constructed using the three sequences along with other Babesia spp. sequences derived from GenBank adopting HKY nucleotide substitution model based on the Bayesian Information Criterion. The phylogenetic analysis confirmed that the three sequences were of Babesia gibsoni and highly divergent from Babesia canis, B. vogeli and B. vulpes. This might be a possible spill over event of B. gibsoni from community dogs through blood feeding dog ticks. This is the first report and molecular confirmation of B. gibsoni infection in captive Indian wolves.
摘要：
保存在ArignarAnna动物园的15只圈养的印度狼（Canis狼疮palipes）的血液样本，Vandalur,对钦奈进行了巴贝虫属的筛查。,通过PCR检测犬埃里希菌和伊凡氏胰蛋白酶的DNA。在15个狼样本中，发现3个样品的巴贝虫属阳性。对3只狼扩增的18SrRNA基因片段进行测序，并确认为gibsoni巴贝斯虫。使用这三个序列以及其他Babesiaspp构建了最大似然树。来自GenBank的序列采用基于贝叶斯信息准则的HKY核苷酸取代模型。系统发育分析证实，这三个序列均为gibsoni巴贝斯虫，与犬巴贝斯虫高度不同，B.vogeli和B.vulpes。这可能是B.gibsoni通过喂血狗蜱从社区狗身上溢出的事件。这是圈养的印度狼中B.gibsoni感染的第一份报告和分子确认。
