Abstract：
OBJECTIVE: To do the etiological analysis of ocular herps virus infection, revealing the pathogen species and the distribution of different virus types within the eye.
METHODS: Samples were collected from 2017 to 2021 at the Department of Ophthalmology, Peking University Third Hospital and tested using real-time PCR for common ocular viruses: herpes simplex virus 1 (HSV-1), cytomegalovirus (CMV), varicella-zoster virus (VZV) and Epstein-Barr virus (EBV). The pathogenesis of the different viruses was classified and analyzed according to the site of infection.
RESULTS: Viral PCR detections were performed on 3627 samples collected over the 5-years and 649 (17.89%) samples contained one or more of the viruses tested. The overall detection rate of CMV was highest at 9.93%. Of all sample types, aqueous humor was the most common (1752 cases), of which 340 were positive (19.41% positive rate). Corneal samples were the next most common, with 1481 cases and 250 positive results (16.88% positive rate). CMV positivity was higher in aqueous humor and corneal samples than other viruses; vitreous body had the highest positive rate at 36.36% (20/55), among which 18 cases were VZV positive.
CONCLUSIONS: Distribution of virus types differed among infection sites, with CMV the most common virus type detected in the cornea and aqueous humor, while VZV was the most common virus detected in the vitreous body.
METHODS: Samples were collected from 2017 to 2021 at the Department of Ophthalmology, Peking University Third Hospital and tested using real-time PCR for common ocular viruses: herpes simplex virus 1 (HSV-1), cytomegalovirus (CMV), varicella-zoster virus (VZV) and Epstein-Barr virus (EBV). The pathogenesis of the different viruses was classified and analyzed according to the site of infection.
RESULTS: Viral PCR detections were performed on 3627 samples collected over the 5-years and 649 (17.89%) samples contained one or more of the viruses tested. The overall detection rate of CMV was highest at 9.93%. Of all sample types, aqueous humor was the most common (1752 cases), of which 340 were positive (19.41% positive rate). Corneal samples were the next most common, with 1481 cases and 250 positive results (16.88% positive rate). CMV positivity was higher in aqueous humor and corneal samples than other viruses; vitreous body had the highest positive rate at 36.36% (20/55), among which 18 cases were VZV positive.
CONCLUSIONS: Distribution of virus types differed among infection sites, with CMV the most common virus type detected in the cornea and aqueous humor, while VZV was the most common virus detected in the vitreous body.
摘要：
目的:进行眼疱疹病毒感染的病原学分析,揭示病原体种类和眼睛内不同病毒类型的分布。
方法：从2017年到2021年在眼科收集样本，北京大学第三医院，采用实时PCR检测常见眼部病毒：单纯疱疹病毒1型（HSV-1），巨细胞病毒（CMV），水痘-带状疱疹病毒（VZV）和EB病毒（EBV）。根据感染部位对不同病毒的发病机理进行分类和分析。
结果：对5年收集的3627个样本进行了病毒PCR检测，649个（17.89%）样本中含有一种或多种所测试的病毒。CMV的总体检出率最高,为9.93%。在所有样品类型中，房水是最常见的（1752例），其中阳性340例(阳性率19.41%)。角膜样本是下一个最常见的样本，1481例，阳性250例（阳性率16.88%）。CMV在房水和角膜标本中的阳性率高于其他病毒；玻璃体的阳性率最高，为36.36％（20/55），其中VZV阳性18例。
结论：感染部位的病毒类型分布不同，CMV是在角膜和房水中检测到的最常见的病毒类型，而VZV是在玻璃体中检测到的最常见的病毒。
方法：从2017年到2021年在眼科收集样本，北京大学第三医院，采用实时PCR检测常见眼部病毒：单纯疱疹病毒1型（HSV-1），巨细胞病毒（CMV），水痘-带状疱疹病毒（VZV）和EB病毒（EBV）。根据感染部位对不同病毒的发病机理进行分类和分析。
结果：对5年收集的3627个样本进行了病毒PCR检测，649个（17.89%）样本中含有一种或多种所测试的病毒。CMV的总体检出率最高,为9.93%。在所有样品类型中，房水是最常见的（1752例），其中阳性340例(阳性率19.41%)。角膜样本是下一个最常见的样本，1481例，阳性250例（阳性率16.88%）。CMV在房水和角膜标本中的阳性率高于其他病毒；玻璃体的阳性率最高，为36.36％（20/55），其中VZV阳性18例。
结论：感染部位的病毒类型分布不同，CMV是在角膜和房水中检测到的最常见的病毒类型，而VZV是在玻璃体中检测到的最常见的病毒。
